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Updated: Jan 14, 2026

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评估直肠拭子是否反映了急性尾炎中的尾微生物群概况:基于16SrRNA的比较研究

Yuan-Yuan Liu¹, Fei Xia², Reyimu Yimuran²

¹Department of Pharmacy, The Seventh Affiliated Hospital of Xinjiang Medical University, Urumqi, 830028, Xinjiang, China.

|January 13, 2026

概括

直肠拭子可能部分反映急性尾炎中尾微生物组的变化,提供一种不那么侵入性的分析方法. 在更大的研究中需要进一步验证.

科学领域:

微生物组研究的研究.
胃肠道的健康状况
传染病诊断传染病诊断传染病诊断

背景情况:

急性尾炎与肠道微生物群的改变有关.
直接尾取样是侵入性的,在健康个体中是不可行的.
研究用于尾炎中微生物组分析的替代方法至关重要.

研究的目的:

为了确定直肠扫描是否可以捕捉成年人急性尾炎的尾微生物群签名.
为了比较尾内含物,直肠拭子和便样本之间的微生物组合和功能.
为了评估尾内容和直肠拭子之间的一致性.

主要方法:

一项涉及急性尾炎患者和健康对照者的前性横截面研究.
收集尾部的内容,手术内直肠拭子,手术后便和对照便.
16S rRNA基因测序和生物信息学分析 (QIIME2,PICRUSt2) 用于评估微生物多样性,组成和功能.

主要成果:

尾含量 (AC) 和直肠拭子 (RS) 显示出比健康对照组 (HC) 更高的遗传学多样性.
与HC相比,AC,RS和手术后便 (IF) 在埃舍里奇亚/西格拉和Fusobacterium中富含,而在丁酸盐生产者中则耗尽.
在配对的AC和RS样本之间观察到基因级和功能一致性,表明RS部分捕捉了尾失生症.

结论:

在外科手术中,直肠拭子可以作为对尾微生物组变化进行分析的最小侵入性辅助.
直肠片可以在属和预测的功能途径水平上部分反映尾失生症.
这些发现需要在更大,更多样化的群体中使用枪头元基因组学来验证.

关键词:

16S rRNA 测序的测序方法急性尾炎是什么情况附属的微生物群.埃舍里希亚/西格拉,菌.我们的肠道微生物群.检查直肠的抽样.

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