相关实验视频

Updated: Jan 14, 2026

Establishment of a Co-culture System of Patient-Derived Colorectal Tumor Organoids and Tumor-Infiltrating Lymphocytes (TILs)

Establishment of a Co-culture System of Patient-Derived Colorectal Tumor Organoids and Tumor-Infiltrating Lymphocytes (TILs)

Published on: June 27, 2025

在结直肠癌中解码先天性淋巴细胞异质性和可塑性

Shuomin Zhang^1,2, Qingfeng Fu^1,3, Zhengyang Xu⁴

¹Medical School of Chinese PLA, Beijing, China.

Clinical and translational medicine

|January 13, 2026

概括

结直肠癌 (CRC) 中的先天性淋巴细胞 (ILC) 的起源和功能各异. 在三级淋巴体结构中的骨髓衍生ILC2s与CRC患者的更好的结果和免疫治疗反应相关.

科学领域:

免疫学免疫学免疫学
癌症生物学癌症生物学
细胞生物学细胞生物学

背景情况:

在结直肠癌 (CRC) 中,先天性淋巴细胞 (ILC) 对于肠道免疫恒温至关重要.
在CRC进展和治疗反应中ILCs的起源和功能尚不清楚.

研究的目的:

阐明ILC子集在结直肠癌微环境中的起源,异质性和功能作用.
调查ILCs对疾病进展和CRC治疗疗效的影响.

主要方法:

单细胞RNA测序 (scRNA-seq) 和空间转录组学 (ST) 用于绘制ILC起源和定位.
在体外共同培养试验和大量RNA测序评估了ILC功能和治疗潜力.
免疫光 (IF) 和流细胞计用于细胞和组织分析.

主要成果:

肠道ILCs来源于胎儿肠道 (ILC3-CD83) 或可能是骨髓 (ILC2,ILC3-S100A4).
在CRC中,ILC3子集分化为应激反应或细胞毒性细胞.
在三级淋巴体结构 (TLS) 中的骨髓衍生ILC2s支持B细胞成熟,与更好的临床结果和免疫治疗反应相关.

结论:

这项研究定义了CRC中肠道ILC子集的独特起源和功能异质性.
丰富的骨髓衍生ILC2s在TLS中与改善CRC的预后和免疫治疗反应有关.
在结直肠癌中,ILC2的丰度可以作为生存和治疗疗效的生物标志物.

关键词:

结肠直肠癌是什么意思天生的淋巴细胞是先天性淋巴细胞.一个单细胞RNA测序.空间转录学空间转录学瘤微环境是一个微环境.

更多相关视频

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Published on: March 23, 2022

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

Published on: September 25, 2011

相关概念视频

Renewal of Intestinal Stem Cells

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Diversity of Antigen Receptors

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...