针切术激活PI3K/Akt信号通路,通过FGF7,10/KDR轴调节细胞亡
Jia-Ming Qian1, Xiao-Le Wang1, Ting Fang1
1Affiliated Hospital of Jiangxi University of Chinese Medicine, Nanchang, Jiangxi, 330006, People's Republic of China.
Journal of pain research
|January 13, 2026
概括
宫脊椎病 (CS) 的针切术治疗通过FGF7,10/KDR信号轴有效调节细胞亡,激活PI3K/Akt通路. 这种机制阐明了针术是如何修复肌肉和治疗CS骨疾病的.
科学领域:
- 整形外科 整形外科 整形外科
- 分子生物学分子生物学
- 传统中国医药 传统中国医药
背景情况:
- 宫脊椎病 (CS) 是一种常见的疾病,治疗机制尚不清楚.
- 针切除术是一种有效的CS临床治疗方法,通常被描述为"调节肌肉以治疗骨疾病".
- 精确的分子机制背后的针术在CS的疗效仍然在很大程度上是未知的.
研究的目的:
- 阐明针切除术在治疗宫脊椎病 (CS) 的分子机制.
- 调查宫肌细胞亡和PI3K/Akt信号通路的作用.
- 探索FGF7,10/KDR信号轴,调解针术对CS的影响.
主要方法:
- 通过X射线和电子显微镜验证的CS大鼠模型的构建.
- 使用免疫光和COIP进行蛋白质相互作用 (FGF7,FGF10,KDR) 的评估.
- 评估运动和感官功能,病理变化和分子表达 (qPCR,西方斑).
主要成果:
- 针切除术改善了CS大鼠的运动和感觉功能,并减少了宫退行.
- 在CS中,FGF7,10/KDR信号轴和PI3K/Akt路径都涉及到CS.
- 针刺术逆转了改变的蛋白质表达,KDR敲击降低了治疗效果.
结论:
- 针切术通过FGF7,10/KDR信号轴激活PI3K/Akt路径.
- 这种激活调节宫肌细胞亡和肌肉修复.
- 这一途径代表了在治疗CS时针切除术的关键分子机制.
相关概念视频
01:22PI3K/mTOR/AKT Signaling Pathway
5.3K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
5.3K
01:17The Extrinsic Apoptotic Pathway
8.0K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
8.0K
01:20The JAK-STAT Signaling Pathway
11.9K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
11.9K
01:31The Intrinsic Apoptotic Pathway
8.3K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
8.3K
01:19Interactions Between Signaling Pathways
7.2K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.2K
01:22Amplifying Signals via Enzymatic Cascade
17.2K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
17.2K