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SPACA4调节精子成熟的结构和分子基础,并最终影响小鼠的精子质量

Xu Chen^1,2, Hai-Qian Wu¹, Dan-Yang Wan^1,3

¹Department of Histology and Embryology, School of Basic Medical Sciences, State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing 211166, China.

Asian journal of andrology

|January 13, 2026

概括

在小鼠中缺少精子形体关联4 (SPACA4) 会导致由于精子形体成熟异常和精子质量受损而导致男性不孕,从而影响生育能力.

科学领域:

生殖生物学生殖生物学
精子生成研究精子生成研究
男性生育能力研究

背景情况:

精子质量对于生殖成功和后代健康至关重要.
精子的成熟是一个复杂的过程,涉及显著的形态变化.
在精子生成中,精子形体相关4 (SPACA4) 的特定作用仍然在很大程度上未知.

研究的目的:

为了研究SPACA4在精子生成过程中的功能.
确定SPACA4缺乏对小鼠精子质量和雄性生育能力的影响.

主要方法:

产生和分析Spaca4淘汰赛小鼠.
使用光和电子显微镜评估精子形态和运动性.
丸基因和蛋白质表达的分析,包括差异蛋白质分析.

主要成果:

SPACA4在小鼠精子和细胞质中得到特定的表达.
Spaca4淘汰赛小鼠表现出男性次生育能力,精子运动率显著降低,形态异常增加.
SPACA4的丧失会扰乱青体,青体和长体的分化,影响核延长,染色质凝聚和鞭毛发育.
在Spaca4-/-精子体中观察到与转化相关的蛋白53目标5 (TRP53TG5) 的表达减少,其淘汰模仿了淘汰现型.

结论:

在小鼠中,SPACA4对于正常的精子成熟和精子质量至关重要.
缺少SPACA4会导致精子容量和体反应受损,最终降低男性生育能力.

关键词:

在SPACA4中使用.多重异常多个异常.精子质量精子质量精子质量精子的成熟过程亚生育能力亚生育能力

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Spermatogenesis

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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...

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Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
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