Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

11.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.7K
Ribosome Profiling02:24

Ribosome Profiling

4.1K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A cross-sectional network analysis of appearance-related anxiety and psychosocial protective factors in adolescent idiopathic scoliosis patients: core symptoms and bridging analysis.

Frontiers in public health·2026
Same author

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Nucleic acids research·2026
Same author

Endoscopic resection or radical gastrectomy? Risk factors for lymph node metastasis in early gastric cancer: a systematic review and meta-analysis.

World journal of surgical oncology·2026
Same author

Two-Dimensional Semiconductors for Postsilicon Electronics: From Transistors to Integrated Circuits.

ACS nano·2026
Same author

The cGAS-STING pathway contributes to cisplatin-induced skeletal muscle atrophy through altered proteostasis and myogenic signaling.

Cell communication and signaling : CCS·2026
Same author

GICP-Based Registration Flow Improvement and Planar Consistency Evaluation for Heterogeneous Multi-LiDAR Systems in Grain Warehousing Robots.

Sensors (Basel, Switzerland)·2026

相关实验视频

Updated: Jan 15, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

5.1K

CircRM:从纳米孔直接RNA测序中分析循环RNA修饰的情况.

Jiayi Li1,2,3, Shenglun Chen1,2,4, Zhixing Wu1,2,5,6

  • 1Department of Biosciences and Bioinformatics, School of Science, Xi'an Jiaotong-Liverpool University, 111 Ren'ai Road, Suzhou Industrial Park, Suzhou, Jiangsu 215123, China.

Briefings in bioinformatics
|January 13, 2026
PubMed
概括

CircRM是一种新的计算工具,使用纳米孔测序对圆形RNA中的RNA修饰进行了分析. 它揭示了圆形RNA的独特修饰模式,与线性RNA不同.

关键词:
圆形的RNARNA是一个圆形的RNA.标志性转录组学 标志性转录组学纳米孔直接RNA测序

更多相关视频

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study
06:57

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study

Published on: July 7, 2023

1.6K
Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.7K

相关实验视频

Last Updated: Jan 15, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

5.1K
Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study
06:57

Author Spotlight: Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study

Published on: July 7, 2023

1.6K
Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.7K

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 循环RNAs (circRNAs) 是关键的调节分子.
  • RNA的修改显著影响circRNA的功能.
  • 现有的方法缺乏解决 circRNA 修饰概况的解决方案.

研究的目的:

  • 开发一种计算方法来分析circRNAs中的RNA修饰.
  • 在单分子分辨率下描述circRNAs的表体转录图形.
  • 为了确定circRNA特定的修饰模式和特征.

主要方法:

  • 开发了CircRM,一种利用纳米孔直接RNA测序的计算工具.
  • 集成的circRNA检测,读取级别修改检测和甲基化速率量化.
  • 应用CircRM来对circRNAs中的三个主要RNA修饰 (m5C,m6A,m1A) 进行分析.

主要成果:

  • 确定了427个高可信度circRNA,并表征了m5C,m6A和m1A的修饰.
  • 与线性RNA相比,在circRNA中揭示了明显的修改模式,在背接接口附近进行丰富.
  • 在不同细胞系中展示了保存和细胞类型特定的修饰模式.

结论:

  • CircRM为研究circRNA修饰提供了一种强大的计算方法.
  • 循环RNA具有独特的表体转录学格局,具有特定于RNA类型的规则.
  • 这些发现有助于进一步了解循环RNA生物学和RNA修饰机制.