Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Plasmids01:28

Plasmids

1.2K
Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...
1.2K
DNA Bacteriophages01:26

DNA Bacteriophages

800
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
800

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Knowledge preservation in the era of big science and AI: strategies for sustainable scientific research.

Nature communications·2026
Same author

Analysis and control of untemplated DNA polymerase activity for guided synthesis of kilobase-scale DNA sequences.

Nature communications·2026
Same author

Using Patient Feedback to Improve Treatment Outcomes for Patients with Congenital Dyserythropoietic Anaemia Type I Receiving Interferon Therapy.

Journal of clinical medicine·2026
Same author

Data-driven inference of digital twins for high-throughput phenotyping of motile and light-responsive microorganisms.

Journal of the Royal Society, Interface·2026
Same author

Emerging trends in genome integration tools for precision engineering of diverse bacterial species.

Synthetic biology (Oxford, England)·2026
Same author

A Decade of SBOL Visual: Growing Adoption of a Diagram Standard for Engineering Biology.

ACS synthetic biology·2025
Same journal

Engineering a Cytochrome P450 <i>O</i>-Demethylase for the Bioconversion of Hardwood Lignin.

ACS synthetic biology·2026
Same journal

Genetic Biosensor for Optimizing Double-Stranded RNA Production by Bacteria.

ACS synthetic biology·2026
Same journal

Heterologous Expression of an Abandoned Termite Mound Fungus Gene Cluster Reveals a Protective Aldehyde-Alcohol Cycle and a Candidate Termiticidal Metabolite.

ACS synthetic biology·2026
Same journal

A Framework for the In Vivo Production of Extensively Engineered Thiopeptides.

ACS synthetic biology·2026
Same journal

A Highly Stringent Split Intein-Mediated DHFR Selectable Marker Enables Efficient Development of High-Producing CHO Cells for Therapeutic Proteins.

ACS synthetic biology·2026
Same journal

Breaking the Stability-Activity-Selectivity Trilemma in Unspecific Peroxygenase through Computation-Based Cross-Regional Combinatorial Mutagenesis.

ACS synthetic biology·2026
查看所有相关文章

相关实验视频

Updated: Jan 15, 2026

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
07:37

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

12.1K

鱼:一个专门的宿主组装R6K等离子体.

Shivang Hina-Nilesh Joshi1, Christopher Jenkins2, David Ulaeto2

  • 1School of Biological Sciences, University of Bristol, 24 Tyndall Avenue, Bristol BS8 1TQ, U.K.

ACS synthetic biology
|January 13, 2026
PubMed
概括
此摘要是机器生成的。

研究人员开发了新的PIR-E. 大肠杆菌菌株,称为SHARK,用于高效的R6K质粒维护和克隆. 这些菌株提高了对具有挑战性的基因组工程项目的克隆效率,使R6K等离子体更容易使用.

关键词:
皮尔品种的皮尔菌株.在R6K等离子体中.克隆,就是克隆人类.有条件的复制.基因组整合 基因组整合羊羔-红色的红色

更多相关视频

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.9K
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

14.8K

相关实验视频

Last Updated: Jan 15, 2026

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
07:37

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

12.1K
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.9K
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

14.8K

科学领域:

  • 分子生物学分子生物学
  • 微生物学 微生物学

背景情况:

  • R6K等离子体对于基因组工程至关重要,但需要特定的PIRE. coli菌株进行维护.
  • 现有的皮尔菌株往往有局限性,包括模糊的遗传背景和低克隆效率.

研究的目的:

  • 为了开发新的E.E. 大肠杆菌菌株优化,以保持稳定的R6K等离子体和高效的克隆.
  • 为开展复杂基因组工程项目的研究人员创建资源.

主要方法:

  • 来自DH10B的SHARK菌株的构建,其中包含一个用于R6K等离子体复制的基因组编码的pir基因.
  • 整合一个 λCI 基因,对特定的等离子体基因进行严格,无条件的抑制.
  • 在大型和复杂的克隆反应中,SHARK菌株在转化效率方面的表现.

主要成果:

  • 与商业Pir菌株相比,Shark菌株的转化效率高出100倍以上.
  • 这些新菌株有助于稳定维护R6K等离子体.
  • 鱼菌株对于克隆大型和复杂的遗传结构是有效的.

结论:

  • 鱼菌株代表了R6K等离子体克隆在基因组工程中的重大进步.
  • 这些菌株解决了现有的皮尔菌株的局限性,提高了克隆效率和可访问性.
  • 鱼菌株和相关工具的公开可用性将使研究界受益.