循环_0042103/TAF15/NER轴调节脉炎的炎症和DNA损伤
Feng Lai1, Jingkun Zhang1, Liecong Lin1
1Department of Endodontics, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, China.
Stem cell research & therapy
|January 14, 2026
概括
循环RNAs通过影响DNA损伤和炎症来调节脉. Circ_0042103与TAF15相互作用,影响DNA修复和恶化脉病的进展.
科学领域:
- 分子生物学分子生物学
- 牙科研究 牙科研究
- 免疫学 免疫学 免疫学
背景情况:
- 循环RNAs (circRNAs) 与炎症性疾病有关.
- 环RNAs在牙炎症 Pulpitis 中的作用仍然在很大程度上是未知的.
- 了解这些作用对于开发新的治疗策略至关重要.
研究的目的:
- 为了研究circRNAs在脉进展中的功能.
- 为了确定参与炎症过程的特定circRNAs.
- 阐明了皮虫中circRNA调节的基础分子机制.
主要方法:
- 微阵列和单细胞RNA测序,以分析脉中DNA损伤反应 (DDR).
- qRT-PCR和西斑用于量化DNA损伤标记物和炎症性细胞因子.
- 功能性测试,以评估circ_0042103对细胞损伤和炎症的影响.
- RNA FISH,拉向和分离试验用于研究circRNA-蛋白相互作用.
- 通过siRNA介导的敲除来评估circ_0042103和TAF15在DNA修复途径中的作用.
主要成果:
- DNA损伤反应 (DDR) 在脉中被激活,与炎症正相关.
- 上调的circ_0042103加剧的脂聚糖 (LPS) 诱导的DDR和牙皮干细胞 (DPSCs) 的炎症.
- Circ_0042103与TAF15相互作用,降低了核酸切除修复 (NER) 蛋白 (ERCC1,PCNA) 的水平,从而增加了DNA损伤和炎症.
结论:
- 通过与TAF15相互作用,Circ_0042103促进了脉,TAF15降低了ERCC1和PCNA的调节.
- 这种相互作用会损害DNA修复,导致人类牙髓干细胞 (hDPSCs) 的DNA损伤和炎症增加.
- 一个新的circ_0042103/TAF15/NER轴被确定为脉病原体的关键参与者.
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