通过准miR-106b-5p/GAB1轴,Circ-PRKCI减轻了败血症继发的急性肺损伤

通过准miR-106b-5p/GAB1轴,Circ-PRKCI减轻了败血症继发的急性肺损伤

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Yingjie Yin¹, Shouqing Zhang², Jiebing Zheng³

¹Department of Critical Care Medicine, The First Affiliated Hospital of Ningbo University, Ningbo, 315010, Zhejiang, China. fyyinyingjie@nbu.edu.cn.

European journal of medical research

|January 14, 2026

概括

循环RNAPRKCI (circ-PRKCI) 通过抑制炎症和亡来减少性肺损伤 (ALI). 它针对miR-106b-5p/GAB1通路,为败血症诱导的ALI提供了潜在的治疗策略.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

败血症引起的急性肺损伤 (ALI) 是死亡的主要原因.
在败血症-ALI中膜上皮细胞损伤的精确分子机制尚未完全理解.
循环RNAs (circRNAs) 参与了败血症-ALI的进展.

研究的目的:

研究circ-PRKCI在与败血症相关的ALI中的功能性作用和潜在机制.
探索circ-PRKCI与microRNA-106b-5p (miR-106b-5p) 和GRB2-关联结合剂1 (GAB1) 的相互作用.

主要方法:

在体外模型中,使用用脂多糖化物 (LPS) 处理的MLE-12细胞来模仿ALI.
在小鼠中通过结和穿孔 (CLP) 诱导的ALI体内模型.
测试包括RT-qPCR,西斑,CCK-8,流细胞计,ELISA,双化酶记者测试和RNA RIP实验.

主要成果:

在体外和体内ALI模型中,Circ-PRKCI显著减轻了亡,炎症和氧化应激.
MiR-106b-5p逆转了circ-PRKCI的保护作用.
环-PRKCI竞争性地与miR-106b-5p结合,从而调节GAB1的表达.

结论:

通过准miR-106b-5p/GAB1轴,Circ-PRKCI可以减轻败血症诱导的ALI.
Circ-PRKCI显示出作为血症次要的ALI的新疗法标的潜力.

关键词:

急性肺损伤是什么?急性肺损伤是什么?周围的PRKCI-PRKCI.在 GAB1 中,GAB1 已被确定为 GAB1 .在MiR-106b-5p中使用.败血症这是一种败血症.

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