长波长斜背照明显微镜用于深度体内成像
Ye-Chan Cho1, Jin Hee Hong1, Sungsam Kang1
1Department of Physics, Korea University, Seoul 02855, Republic of Korea.
Biomedical optics express
|January 14, 2026
概括
斜背照明显微镜 (OBM) 现在使用更长的1650纳米波长来克服分散,并实现更深的 in vivo 大脑成像在小鼠. 这种无标签的技术增强了对生物系统的可视化,用于先进的研究.
科学领域:
- 生物医学光学 生物医学光学
- 显微镜的使用方法
- 在生物体内成像成像.
背景情况:
- 斜背照明显微镜 (OBM) 是一种无标签的成像方法.
- 在反射模式下,OBM通过检测向前散射的光来产生高对比度图像.
- 组织散射限制了OBM在生物组织中的成像深度.
研究的目的:
- 为了扩大OBM的成像深度.
- 为了减轻生物组织中的散射效应.
- 开发一个长波长的OBM系统,用于更深的体内成像.
主要方法:
- 实施了一种新型的OBM系统,在1650nm波长运行.
- 我们将1650nm的OBM系统与800nm系统进行了比较,用于体内小鼠大脑成像.
- 在生物组织中评估成像深度和对比度.
主要成果:
- 与800纳米系统相比,1650纳米的OBM系统实现了 significantly更深的老鼠大脑体内成像.
- 较长的波长有效地减轻了散射效应,允许更深的透.
- 高对比的伪传输图像在较深的深度获得.
结论:
- 长波长的OBM (1650nm) 显著提高了在分散生物组织中的成像深度.
- 这一进步有助于对老鼠大脑进行更深入的体内研究.
- 开发的OBM系统为对生物系统及其病理生理学的详细研究提供了潜力.
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