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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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使用周期结构化照明进行电压成像.

Forest Speed1, Alec Teel2, Timothy Young1

  • 1Department of Bioengineering, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.

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概括
此摘要是机器生成的。

使用伪HiLo (pHiLo) 图像重建的周期结构化照明可以减少电压成像中的失焦信号. 与传统的广场记录相比,这种方法可以提高对焦细胞的信号清晰度.

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科学领域:

  • 神经科学是一个神经科学.
  • 光学成像技术的成像
  • 生物物理学的生物物理.

背景情况:

  • 在体内电压成像对于理解神经电路动态至关重要.
  • 失焦的蜂信号可能会污染记录,降低电压活动测量的准确性.
  • 传统的广场成像和伪广场 (pWF) 重建难以有效地减轻这种失焦光.

研究的目的:

  • 评估周期结构化照明的有效性,使用伪HiLo (pHiLo) 图像重建进行体内电压成像.
  • 为了比较pHiLo与传统广场录制和伪广场 (pWF) 重建在减少失焦信号方面的性能.
  • 评估pHiLo对关键成像指标的影响,例如尖峰峰与噪声比率 (PNR) 和细胞背景相关性.

主要方法:

  • 使用周期结构化照明与伪HiLo (pHiLo) 图像重建.
  • 进行了体内电压成像实验.
  • 将pHiLo与传统的广场照明和伪广场 (pWF) 重建进行比较.
  • 分析了尖峰峰与噪声比率 (PNR) 和细胞背景相关性,用于时间课程.

主要成果:

  • 与传统的广场记录和pWF重建相比,pHiLo显著减少了失焦细胞的信号.
  • 通过使用pHiLo.Lo,证明了对使用pHiLo.Lo感兴趣的聚焦电池的电压活动的提高清晰度.
  • 在pHiLo和索马向的广场照明 (TI) 之间进行了PNR和细胞背景相关性的定量比较.

结论:

  • pHiLo是一种有效的技术,用于降低在高速度体内电压成像中的失焦光污染.
  • 这项研究突出了pHiLo.lo的失焦光减少,信号噪声比和时间分辨率之间的权衡.
  • 通过提高信号质量,pHiLo提供了在清醒的小鼠中成像神经活动的优势.