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相关概念视频

CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Conservative Site-specific Recombination and Phase Variation02:53

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Updated: Jan 15, 2026

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
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在Streptococcus agalactiae17型多部位序列菌株中的CRISPR干扰.

William D Cutts1, Aidan W Flanagan2, Brice K Gorman2

  • 1Department of Molecular and Cell Biology, University of Texas at Dallas, Dallas, Texas, USA.

Journal of bacteriology
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PubMed
概括

研究人员开发了一种CRISPR干扰 (CRISPRi) 系统,用于研究B组链球菌 (GBS) 病原性. 这种工具可以在高毒性ST-17菌株COH1中进行向基因淘汰,帮助研究新生儿脑膜炎.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?B组的链球菌.

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科学领域:

  • 微生物学 微生物学
  • 遗传学 是一个遗传学.
  • 传染性疾病 传染性疾病

背景情况:

  • 乙组链球菌 (GBS) 是新生儿细菌性脑膜炎的主要原因.
  • 高病毒性血清型III,序列型17 (ST-17) 菌株COH1与严重疾病有关,但在遗传学上很难操纵.
  • 了解血脑屏障 (BBB) 中的GBS毒性因素对于对抗新生儿脑膜炎至关重要.

研究的目的:

  • 开发一种新型的CRISPR干扰 (CRISPRi) 系统,用于针对GBS ST-17 COH1菌株的向基因敲除.
  • 为了使功能基因组学和高通量查GBS毒性因子.
  • 在BBB调查GBS相互作用.

主要方法:

  • 在COH1菌株中使用催化不活化的Cas9 (dCas9) 开发CRISPR干扰 (CRISPRi) 系统.
  • 通过血液溶解试验和qPCR转录分析确认系统疗效.
  • 用人脑内皮细胞感染模型在体外评估基因淘汰效应.

主要成果:

  • 在ST-17 GBS中,CRISPRi系统成功实现了可调的基因表达敲除.
  • 观察到关键毒性基因 (PI-2b, srr2, iagA) 的表型突破.
  • 在BBB表现出减少的细菌粘附,入侵和炎症反应.

结论:

  • 开发的CRISPRi平台为ST-17 GBS的基因操纵提供了一个多功能工具.
  • 该系统为GBS提供了快速的功能基因组学和病变发生学研究.
  • 这些发现有助于更好地了解GBS毒性和潜在的治疗点.