使用Auramine O进行Bacillus subtilis 168成熟子的流动细胞测量区分
Yuka Harada1, Ritsuko Kuwana2, Shoko Yagi1
1Research Division, TOA Biopharma Co., Ltd., Tatebayashi, Gunma, Japan.
Bioscience, biotechnology, and biochemistry
|January 14, 2026
概括
一种新的方法使用Auramine O (AuO) 染色和流动细胞计 (FCM) 来轻松识别Bacillus subtilis子. 在55°C加热子可增强光染色,以便精确的细菌计数.
科学领域:
- 微生物学 微生物学
- 分析化学 分析化学
- 生物技术是生物技术.
背景情况:
- 准确的细菌子计数对于食品安全和益生菌制造至关重要.
- 在 Bacillus subtilis 种群中,区分成熟子与植物细胞可能是一项挑战.
研究的目的:
- 开发一种简单,快速和有效的方法来区分成熟的Bacillus subtilis168子和混合细胞种群.
- 为了优化子染色和识别,使用奥拉O (AuO) 和流细胞计 (FCM).
主要方法:
- 使用的Auramine O (AuO) 为Bacillus subtilis 168.8进行光染色.
- 采用流细胞计 (FCM) 分析来测量光强度.
- 通过在不同温度 (25,40,55,70°C) 进行30分钟的热处理测试,优化了染色.
主要成果:
- 奥拉胺O (AuO) 首选染色成熟的子,使得基于光的歧视.
- 在55°C温度下加热30分钟,使子与其他细胞类型分离得最明显.
- 优化的方法通过FCM证明了有效和准确的子识别.
结论:
- 与FCM结合的AuO染色和热处理提供了一种高通量,可扩展的子识别方法.
- 这种技术对于需要快速可靠的细菌计数的应用非常有价值,例如食品安全和益生菌生产.
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