相关实验视频

Updated: Jan 17, 2026

Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation

Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation

Published on: August 19, 2020

细胞衍生的外生体指导树突细胞依赖的CD8⁺ T细胞激活和增殖在脏炎症

Bin Qian¹, Shuya Mao², Yang Chen³

¹State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, School of Life Science and Technology, China Pharmaceutical University, 639 Longmian Avenue, Nanjing, Jiangsu 211198, China; National Clinical Research Center for Kidney Diseases, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, Jiangsu 210002, China.

Journal of advanced research

|January 14, 2026

概括

细胞衍生的外生体在最小变化疾病 (MCD) 中激活T细胞,导致脏损伤. 通过Rab27a抑制外体分泌为MCD提供了潜在的新疗法.

科学领域:

免疫学免疫学免疫学
腎臟病學 (nephrology) 是一種醫學.
细胞生物学细胞生物学

背景情况:

最小变化疾病 (MCD) 导致瘤综合征,其中涉及细胞损伤和T细胞激活.
现有的知识差距表明MCD免疫病原发生的未发现机制,特别是关于T细胞相互作用.

研究的目的:

通过MCD的抗原呈现来激活T细胞的podocyte衍生外体 (Pdo-Exos) 的作用.
探索Pdo-Exos分泌的机制及其对疾病进展的影响.

主要方法:

使用了体外共同培养系统,一种胺氨核酸酸 (PAN) 诱导的MCD小鼠模型和人类临床样本.
评估了Pdo-Exos的免疫刺激能力,重点关注MHC-I表现和Rab27a调节的外体分泌.

主要成果:

通过MHC-I,Pdo-Exos通过MHC-I激活了CD8+ T细胞,这表明树突细胞交叉呈现.
在小鼠模型中,Pdo-Exos恶化了蛋白尿和T细胞激活;MCD患者表现出循环激活T细胞的增加,Pdo-Exos增加了MHC-I和CD80.
Rab27a依赖的外体分泌与疾病严重程度相关,而其抑制减少了蛋白尿和炎症.

结论:

细胞衍生外体作为抗原呈现囊泡,调解CD8+T细胞激活,树突细胞促进T细胞扩张.
Rab27a-依赖的外体分泌是MCD进展的关键因素.
针对Rab27a介导的外体释放,为MCD提供了一个新的治疗策略.

关键词:

抗原呈现方式疾病变化最小的变化由细胞衍生的外体细胞.在 Rab27a 中,在T细胞激活过程中,

更多相关视频

Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection

Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection

Published on: November 10, 2021

Reliable and High Efficiency Extraction of Kidney Immune Cells

Reliable and High Efficiency Extraction of Kidney Immune Cells

Published on: August 19, 2016

相关概念视频

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...