青少年C57BL/6小鼠在产前酒精暴露后发生的性别特异性肠道微生物群变化
Simone Baldi1, Sara Bertorello1, Francesco Cei1
1Department of Experimental and Clinical Medicine, University of Florence, 50134 Florence, Italy.
概括
产前酒精暴露 (PAE) 破坏了青少年小鼠的肠道微生物群,特别是男性,影响微生物多样性和短链脂肪酸. 这些发现表明,对于胎儿酒精谱系障碍 (FASD) 的性别特异性微生物向干预措施.
科学领域:
- 神经科学是一个神经科学.
- 微生物学 微生物学
- 发育生物学 发展生物学
背景情况:
- 胎儿酒精谱系障碍 (FASD) 是产前酒精暴露 (PAE) 的结果,导致终身的身体,认知和行为缺陷.
- 人们越来越多地认识到肠道微生物组在FASD病变发生中的作用,但性别特异性的影响仍未得到充分研究.
研究的目的:
- 研究早期PAE对青少年小鼠肠道微生物组和代谢组的影响.
- 确定特定于性别的变化和FASD早期干预的潜在目标.
主要方法:
- 雌性小鼠在怀孕期间接受了乙醇或水;后代在青少年时被分析.
- 用16S rRNA测序来评估肠道微生物组的组成.
- 代谢分析分析使用气相色谱-质谱学识别了短链脂肪酸 (SCFA).
主要成果:
- PAE显著降低了肠道微生物的α多样性,并改变了后代的β多样性模式.
- PAE小鼠的便酸盐增加和抗炎性SCFA减少;雄性表现出更明显的干扰.
- 在PAE男性中,一个失生子组表现出严重的多样性丧失,促炎性种类丰富,以及改变的代谢途径.
结论:
- 早期的PAE在青少年小鼠中诱导性别和个体特定的肠道失调.
- 微生物组的改变,特别是在男性中,突出了FASD的潜在治疗点.
- 需要进一步的研究来开发针对性,基于微生物群的FASD干预策略.
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