细胞结点mRNAs的局部调节对于上皮细胞完整性是必要的
Ashley Chin1, Jonathan Bergeman1, Laudine Communal2
1Institut de recherches cliniques de Montreal.
概括
在细胞结处局部化的mRNA对于上皮细胞的极性和完整性至关重要. MAGOH和ZO-1的表达与卵巢癌的良好预后相关,这表明它们作为生物标志物的潜力.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 表皮细胞依赖于基极性来起作用,这种过程在癌症中经常被破坏.
- 亚细胞mRNA局部化是影响表皮细胞极性的一种尚未研究的机制.
研究的目的:
- 研究mRNA局部化对细胞结点在维持上皮质极性中的作用.
- 为了识别参与在细胞结合处向和翻译mRNA的RNA结合蛋白.
- 探索卵巢癌中已确定的因素的临床相关性.
主要方法:
- 在Drosophila和人类上皮细胞中进行免疫标记,以识别结点mRNA和蛋白质.
- 查RNA结合蛋白和功能测定 (功能丧失).
- 在体内研究Drosophila和组织微阵列分析人类卵巢癌样本.
主要成果:
- 极性蛋白的mRNAs (Zo-1,Afdn,Scrib) 局部化到细胞结点,具有同局部化翻译.
- 几种RNA结合蛋白与结节转录相关,对ZO-1和SCRIB向至关重要.
- MAGOH 枯竭会影响转录的定位和翻译;PCBP3 枯竭只会影响本地翻译.
- 在Drosophila中MAGO的枯竭破坏了结节转录和蛋白质分布.
- MAGOH和ZO-1表达在卵巢癌中具有积极的相关性,表明潜在的预后价值.
结论:
- 在细胞结处局部化的mRNA调节对于上皮细胞完整性至关重要.
- MAGOH和ZO-1可以作为卵巢癌的良好预后的生物标志物.
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