相关实验视频

Updated: May 5, 2026

Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment

Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment

Published on: September 11, 2014

多omics分析Dhx37缺乏对丸发育和核细胞平衡的影响

Yuqing Jiang¹, Jiali Chen¹, Yanshuang Ren¹

¹Henan Key Laboratory of Rare Diseases, Endocrinology and Metabolism Center, The First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and Technology, Luoyang, China.

Cell death discovery

|January 14, 2026

概括

DHX37对于塞尔托利细胞生存和丸发育至关重要. 它的损失破坏了核细胞的完整性,导致apoptosis和小鼠的精子生成受损.

科学领域:

生殖生物学生殖生物学
分子细胞生物学分子细胞生物学
遗传学和基因组学遗传学和基因组学

背景情况:

塞尔托利细胞对于丸微环境中的精子生成至关重要.
DHX37是一种RNA螺旋酶,与46种XY性发育障碍 (DSD) 有关.
DHX37在塞尔托利细胞功能和丸发育中的确切作用需要阐明.

研究的目的:

研究DHX37在维持塞尔托利细胞存活率和丸完整性的功能.
阐明DHX37缺乏影响丸发育的分子机制.

主要方法:

RNA测序 (RIP-seq,RNAi-RNA-seq,snRNA-seq) 用于分析基因表达和拼接.
生成和分析细胞特异的Dhx37淘汰赛小鼠 (Dhx37^-/-).
免疫光染色,qRT-PCR,以及西式涂抹,以验证分子发现.

主要成果:

失去DHX37会破坏细胞核的完整性,抑制PI3K-AKT信号传递,并通过亲细胞结触发p53驱动的亡.
Dhx37^-/-小鼠表现出 reduced 丸体积,较低的丸激素,精子管的真空化,以及增加的亡.
基因表达分析显示,在Dhx37缺乏的丸中,亡和增殖途径发生了显著的改变.

结论:

DHX37是小鼠塞尔托利细胞中核结构的关键决定因素.
通过DHX37维持核细胞完整性对于正常的丸形态发生和精子发生是必不可少的.
缺乏DHX37会损害塞尔托利细胞的功能,导致丸发育失败.

更多相关视频

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

Published on: August 20, 2019

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish

Published on: January 28, 2020

相关概念视频

Dosage Compensation

Dosage Compensation

In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...

Exon Recombination

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...