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相关概念视频

Epigenetic Regulation01:46

Epigenetic Regulation

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Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
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Epigenetic Regulation01:37

Epigenetic Regulation

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Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
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相关实验视频

Updated: Jan 17, 2026

Massively Parallel Reporter Assays in Cultured Mammalian Cells
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使用e2MPRARA同时进行表观基因组分析和监管活动测量.

Zicong Zhang1, Ilias Georgakopoulos-Soares2, Guillaume Bourque1,3,4,5

  • 1Institute for the Advanced Study of Human Biology (WPI-ASHBi), Kyoto University, Kyoto, Japan.

Nature communications
|January 14, 2026
PubMed
概括
此摘要是机器生成的。

我们开发了e2MPRA,这是一种新方法,可以同时分析cis-regulatory元素 (CREs) 的调节功能和表观基因组修饰. 这种技术有助于对基因组的理解.

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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
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相关实验视频

Last Updated: Jan 17, 2026

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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
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科学领域:

  • 基因组学就是基因组学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 分子生物学分子生物学

背景情况:

  • 基因组范围内的cis-regulatory元素 (CREs) 标注依赖于转录因子 (TF) 结合和基因组修饰的描述性测试.
  • 对CREs的功能验证至关重要,但具有挑战性,目前没有技术能够同时分析调节功能和表观基因组状态.

研究的目的:

  • 开发一种新技术,同时对CRE调节功能和相关的表观基因组修饰进行高通量分析.
  • 为了使合成增强剂内TF动机的功能剖析,并评估序列扰乱对表观遗传状态的影响.

主要方法:

  • 开发一种丰富,然后进行表观基因组分析的大规模并行报告员测试 (e2MPRA).
  • 使用lentivirus进行CRE丰富和集成,并与MPRA,Cut&Tag或ATAC-seq.相结合.
  • 允许同时评估调节活性,蛋白质结合和表观遗传修饰.

主要成果:

  • 证明了e2MPRA在合成增强剂中剖析TF动图的表观遗传功能的能力.
  • 通过使用e2MPRA成功评估了序列扰乱对表观遗传状态的影响.
  • 实现了对特定序列的调节功能和表观基因组修饰的同时高通量分析.

结论:

  • e2MPRA是一种强大的新技术,用于对cis-regulatory元素进行全面分析.
  • 该方法促进了对监管代码的理解,对表观基因组的影响,以及改变的表型后果.