天然的机制及其有效成分对抗性结肠炎
Ke-Yu Ma1, Bao-Long Hou1, An-Qi Fan2
1Co-construction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi and Education Ministry, State Key Laboratory of Research, Shaanxi University of Chinese Medicine, Xianyang, 712083, China.
Scientific reports
|January 14, 2026
概括
天然 (IN) 和其成分通过调节炎症途径,显示出治疗性结肠炎 (UC) 的潜力. 这项研究阐明了IN背后的分子机制.
科学领域:
- 传统中国医药 传统中国医药
- 药理学 药理学是指药理学的学科.
- 免疫学 免疫学 免疫学
背景情况:
- 天然 (IN) 在中国传统医学中已有很长的使用历史.
- IN因其抗炎性质而闻名,特别是在性结肠炎 (UC) 中.
- 在UC中IN治疗作用的精确分子机制尚不清楚.
研究的目的:
- 阐明Indigo Naturalis (IN) 在治疗性结肠炎 (UC) 中的分子机制.
- 为了研究IN及其活性成分 (青,无素,异素,MIX) 在体外和体内炎症途径的影响.
主要方法:
- 在体外:用脂聚糖 (LPS) 诱导炎症的RAW264.7细胞;用于细胞活力和抗炎活性的MTT和NO测定;用于细胞因子表达的ELISA;用于MAPK,STAT3和NF-κB通路的西部斑点.
- 在体内:C57BL/6小鼠的DSS诱导的性结肠炎 (UC) 模型;使用疾病活性指数 (DAI),髓氧化酶 (MPO) 和H&E染色进行评估.
主要成果:
- IN,因德鲁宾和异氨酸证明了对炎症性细胞因子表达的调节.
- IN的机制涉及MAPK,STAT3和NF-κB通路;因德鲁抑制NF-κB和STAT3;异氨酸调节NF-κB和MAPK.
- 在MAPK中抑制STAT3激活和p-P38表达,显著改善UC小鼠模型.
结论:
- 天然及其活性成分,特别是MIX,对性结肠炎具有显著的治疗潜力.
- 抗炎作用通过调节关键信号通路,包括STAT3和MAPK,进行调节.
- 了解这些分子机制为开发基于IN的UC治疗提供了基础.
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