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NtMYB308 负面调节安托素和素的生物合成,调节尼古提亚烟草的真菌抵抗力

Nivedita Singh¹, Shambhavi Dwivedi¹, Deeksha Singh¹

¹Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow, India.

Plant, cell & environment

|January 14, 2026

概括

NtMYB308在烟草中起到抑制作用,控制了素和素的产生. 它的操纵为增强植物抗病能力和促进可持续农业提供了一条途径.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
生物化学生物化学

背景情况:

安西氨酸和氨酸是关键的烯胺代谢物,参与植物防御和发育.
R2R3-MYB转录因子调节这些通路,作为激活剂或抑制剂.
NtMYB308是一种烟草R2R3-MYB因子,具有已知的抑制域.

研究的目的:

为了功能性地描述NtMYB308在调节烟草中的甲代谢中的作用.
为了研究NtMYB308对素和素生物合成的影响.
评估NtMYB308对植物疾病耐药性的影响.

主要方法:

病毒诱导的基因沉默 (VIGS) 以减少NtMYB308的表达.
编辑CRISPR/Cas9基因以创建淘汰 (NtMYB308CR) 和过度表达 (NtMYB308OX) 线.
促进剂结合测试以确定转录活性.

主要成果:

NtMYB308 作为一种转录抑制剂,可以抑制安东素和素生物合成基因的作用.
植物的NtMYB308CR表现出增加的素和素,增强的真菌耐药性.
NtMYB308OX植物表现出减少的素和素,增加对病原体的敏感性.

结论:

NtMYB308是烟草中烯胺代谢的关键负调节剂.
调节NtMYB308可以增强植物的抗病能力和抗氧化能力.
准NtMYB308为可持续农业和减少农药使用提供了机会.

关键词:

它是AtMYB4型的.MYB3088,这是一个很好的方法.安托西安尼尼 (anthocyanin) 是一种生物压力是生物压力.基因表达的基因表达方式基因组编辑-基因组编辑红色的线性蛋白质 (lignin) 是一种负面监管是一种消极的监管.烟草烟草的烟草烟草是一种烟草.

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