直接注射质谱和iFishMass用于抗体修饰的高通量分析.
Jennifer Aguilan1, Carlos Madrid-Aliste2,3, Fereshteh Zandkarimi4
1Department of Pathology, Albert Einstein College of Medicine, Bronx, New York, New York 10461, United States.
ACS pharmacology & translational science
|January 15, 2026
概括
一个新的质谱仪 (MS) 协议可以在30秒内快速,定量地分析改性. 这种高通量平台通过有效选抗体药物联合体 (ADC) 等蛋白质上的结合位来加速药物开发.
科学领域:
- 分析化学 分析化学
- 化学生物学 化学生物学
- 生物化学 生物化学
背景情况:
- 高通量选对于药物开发至关重要,需要对大型化合物库进行高效分析.
- 基于质谱 (MS) 的蛋白质组学对于识别和量化蛋白质修饰,包括翻译后修饰 (PTM) 是至关重要的.
- 抗体药物联合体 (ADC) 的兴起需要更快的方法来分析蛋白质修饰.
研究的目的:
- 提出一种新的,用于对改性的定量分析的快速协议.
- 开发一个高通量平台,用于选蛋白质上的结合位,特别是ADC.
- 引入新的软件 (iFishMass) 进行高效的数据提取和分析.
主要方法:
- 直接注射MS方法与一种新的软件iFishMass相结合.
- 在大约30秒的MS时间内获取修饰的定量数据.
- 使用多通道管管和96孔板,对最多384个样本进行并行样本制备.
主要成果:
- 获得了改性的定量数据,MS的快速获取时间为~30秒.
- 开发了iFishMass软件,用于自动提取目标信号并从多个运行中生成图表/统计数据.
- 已证明并行样本处理的潜力,使高通量选具有成本效益.
结论:
- 提出的平台可以显著更快地分析ADC开发的单克隆抗体上的合成修饰.
- 该协议对于分析生物翻译后修饰 (PTMs) 具有潜在的可扩展性.
- 这种廉价而有效的平台可促进蛋白质结合部位的高通量选.
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