从具有侵入性肺阿斯伯吉洛症和其他菌感染的免疫受损患者的呼吸和活检样本中进行粘膜PCR检测:来自多中心ECMM研究的结果
Sarah Sedik1, Robina Aerts2,3, Katrien Lagrou2,3
1Department of Internal Medicine, Division of Infectious Diseases, Medical University of Graz, Translational Mycology Research Center, Excellence Centre for Medical Mycology (ECMM), Graz, Austria.
在MucorGenius® PCR测定显示在检测Mucorales DNA方面具有很高的灵敏度,改善了高风险患者的粘菌菌病和共感染的诊断. 这种分子测试有助于在传统方法失败时识别侵入性菌感染.
科学领域:
- 医学真菌学 医学真菌学
- 分子诊断学 分子诊断学
- 传染性疾病 传染性疾病
背景情况:
- 菌病的诊断是具有挑战性的,因为传统方法的敏感性较低,血清学测试缺乏.
- 对于疑似侵入性菌感染 (IMI) 的高风险患者来说,早期和准确的诊断至关重要.
研究的目的:
- 评估MucorGenius® PCR测定用于检测Mucorales DNA的性能.
- 评估该试验在呼吸道和活检样本中诊断粘膜菌和其他IMI的实用性.
主要方法:
- 一项多中心队列研究,涉及4个地点的114名疑似IMI患者.
- 使用MucorGenius® PCR测定对132个呼吸道和活检样本的分析.
- 将PCR结果与标准真菌诊断方法进行比较.
主要成果:
- 在28%的样本中检测到菌DNA (37/132),包括已证明的菌菌病和侵入性肺阿斯伯吉洛症 (IPA) 的病例.
- 该试验显示了94.4%的可能/已被证明的粘膜菌的灵敏度,确定了11种常规诊断中错过的共感染.
- 在可能患有IMI和IPA的患者中也检测到了粘菌DNA,这突显了其广泛的实用性.
结论:
- 在MucorGenius® PCR测定提供了高灵敏度的Mucorales检测,潜在地改善粘菌的诊断.
- 这种测试对于识别Aspergillus-Mucorales联合感染和诊断常规方法呈阴性结果时的粘膜菌非常有价值.
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