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相关概念视频

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In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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相关实验视频

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Rasd2 Knockout 夸大了 Tsc1 缺陷小鼠的听力损失表型.

Rui Ren1,2, Haiyue Xing1, Xiaoying Wang1

  • 1Shandong Provincial Key Laboratory of Development and Regeneration and Key Laboratory for Experimental Teratology of the Ministry of Education, School of Life Sciences, Shandong University, Qingdao, Shandong, China.

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内耳中的蛋白质RASD2不影响单独的听力,但与TSC1缺乏结合时会使听力损失恶化. 这表明RASD2调节了mTOR活动和立体形态学,用于正常听力.

关键词:
在RASD2中,RASD2是RASD2的第二种类型.这就是TSC1的特点.头发细胞 头发细胞 头发细胞内耳的内耳是内耳的内耳.在 mTOR 路径上.立体是什么? 立体是什么

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科学领域:

  • 内耳生物学 内耳生物学
  • 分子遗传学 分子遗传学
  • 听觉神经科学 听觉神经科学

背景情况:

  • Rasd2 (Ras相关的Dexras1) 是一种带状体丰富的转录,涉及到mTOR信号传递.
  • Rasd2也存在于内耳毛细胞中,但其作用尚不清楚.
  • TSC1是mTOR信号的负调节者,其缺乏会导致听力损失.

研究的目的:

  • 研究Rasd2在内耳中的作用及其对听力的贡献.
  • 确定Rasd2是否与TSC1相互作用,以调节毛细胞功能和听觉感知.

主要方法:

  • 使用CRISPR/Cas9.9生成Rasd2淘汰赛小鼠.
  • 产生的Rasd2/Tsc1双淘汰赛小鼠. 产生的Rasd2/Tsc1双淘汰赛小鼠.
  • 分析了头发细胞立体细胞形态和听觉功能.
  • 在耳组织中评估mTORC1通路激活.

主要成果:

  • 单独的Rasd2淘汰赛并没有影响听觉功能或立体眼睛.
  • 在 Tsc1 缺陷小鼠中,Rasd2 淘汰会加剧听力损失.
  • 双击淘汰的小鼠表现出严重的立体缺陷和增强的mTORC1激活.

结论:

  • RASD2在维持立体形态和听觉功能方面发挥着至关重要的作用.
  • RASD2可能调节mTOR活动,有助于听力传导.
  • RASD2和TSC1之间的相互作用对于正常的内耳功能至关重要.