接近红外的同晶纳米光体,具有增强的两光子吸收截面
Liangmeng Hao1, Ying Ni1, Jiawei Huang2
1State Key Laboratory of Advanced Materials for Intelligent Sensing, Key Laboratory of Organic Integrated Circuits, Ministry of Education, Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|January 15, 2026
概括
研究人员开发了新的有机纳米粒子 (BP4TC-NP) 用于先进的生物成像. 这些纳米粒子有效地吸收近红外光以产生深红色光,为低细胞毒性多光子成像提供了一个有前途的工具.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术 纳米技术
- 生物光子学 生物光子学
背景情况:
- 有机纳米探测器具有宽带两光子吸收 (TPA) 和近红外 (NIR) 激发对于生物成像和光子学至关重要.
- 设计和准备这些材料带来了重大挑战.
研究的目的:
- 开发一种具有宽带TPA和NIR光学激发的新型有机纳米探针材料.
- 描述合成材料的TPA特性和生物适用性.
主要方法:
- 同结晶策略合成BP4TC (捐赠者BP4VA,接受者TCNB).
- 纳米沉制造可在水中分散的BP4TC纳米颗粒 (BP4TC-NP).
- 开放孔Z扫描测量用于TPA截面测定.
- 第一个原则计算和TDDFT方法用于理论验证.
- 使用A549细胞 (人类肺癌细胞) 进行细胞内成像.
主要成果:
- BP4TC-NP表现出700-1000nm的宽带TPA特征,TPA截面正在减少.
- 对于一个分子多元件固体,TPA截面的第一次实验性确定.
- 第一原则计算证实了增强的分子间电荷转移和TPA能力.
- 在900nm激发下,在A549细胞中观察到明亮的低背景光.
- 对于BP4TC-NP证明了微不足道的细胞毒性.
结论:
- BP4TC-NP是一种强大的两光子吸收器,具有宽带NIR-I TPA和有利的生物特性.
- 开发的共晶材料作为生物多光子成像和NIR光子学的多功能平台.
- 为设计先进的非线性光学纳米材料提供了经过验证的蓝图.
相关概念视频
Super-resolution Fluorescence Microscopy
12.2K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.2K
Total Internal Reflection Fluorescence Microscopy
11.0K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.0K
Fluorescence and Phosphorescence: Instrumentation
1.4K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.4K


