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相关概念视频

Combinatorial Gene Control02:33

Combinatorial Gene Control

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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Somatic to iPS Cell Reprogramming01:29

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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Methods of Nuclear Reprogramming01:24

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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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相关实验视频

Updated: Jan 18, 2026

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
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一个用于免疫细胞重编程的组合转录因子选平台.

Ilia Kurochkin1, Abigail R Altman1, Inês Caiado2

  • 1Molecular Medicine and Gene Therapy, Science for Life Laboratory, Lund Stem Cell Center, Lund University, BMC A12, 221 84 Lund, Sweden; Wallenberg Centre for Molecular Medicine, Lund University, BMC A12, 221 84 Lund, Sweden.

Cell systems
|January 15, 2026
PubMed
概括
此摘要是机器生成的。

研究人员开发了REPROcode,这是一个用于免疫细胞重编程的平台. 该工具识别了转录因子 (TF) 组合,用于为先进的免疫疗法应用设计免疫细胞.

关键词:
呈现抗原的细胞.条码编码 条码编码细胞重新编程的细胞重编程.结合性选检查是一种综合性选.树突细胞是一种树突细胞.天生的淋巴细胞是先天性淋巴细胞.机器学习是机器学习.转录因子的转录因子转录的层次结构.

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Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
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Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
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Last Updated: Jan 18, 2026

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
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Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

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Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
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科学领域:

  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学
  • 生物工程是生物工程.

背景情况:

  • 免疫细胞的直接重编程具有治疗潜力,但由于对转录因子 (TF) 网络的不完全理解而受到阻碍.
  • 确定有效的TF组合对于推进免疫疗法策略至关重要.

研究的目的:

  • 开发和验证REPROcode,这是一个新的平台,用于TFs的组合查,以确定用于免疫细胞重编程的组合.
  • 探索免疫TF的更广泛的重编程能力,并构建TF等级地图.

主要方法:

  • 开发REPROcode,一个使用多重TF集的单细胞选平台.
  • 构建和选一个配列的lentiviral库的408条码免疫TFs.
  • 通过诱导特定的免疫细胞类型,如cDC1s和NK类细胞来验证REPROcode.

主要成果:

  • REPROcode成功地确定了cDC1诱导的最佳TF静脉测量,忠实度增强剂和调节剂.
  • 查显示了髓状和淋巴状表型,导致创建TF等级图.
  • 该平台通过成功诱导类似自然杀手 (NK) 细胞来证明其发现能力.

结论:

  • REPROcode是一个多功能工具箱,用于工程免疫细胞,显著推进免疫疗法研究.
  • 这项研究增强了对免疫转录控制和TF网络的理解.
  • 开发的TF层次地图为未来免疫细胞重编程工作提供了指导.