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相关概念视频

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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整合基于成像和基于测序的空间奥米克在同一组织部分的映射,通过DBiTplus进行映射.

Archibald Enninful1, Zhaojun Zhang2, Dmytro Klymyshyn3

  • 1Department of Biomedical Engineering, Yale University, New Haven, CT, USA.

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组织测序加 (DBiTplus) 中的确定性条形编码集成了空间转录和蛋白质成像. 这种多式模式的方法绘制了组织中的细胞和途径的地图,促进了对细胞异质性和疾病机制的理解.

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科学领域:

  • 空间奥米克斯 空间奥米克斯
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 了解细胞异质性和功能需要在组织架构中映射分子数据.
  • 目前的空间奥米克斯方法往往缺乏多模式集成或单细胞分辨率.

研究的目的:

  • 介绍组织测序加 (DBiTplus) 中的确定性条形编码,这是一个集成的多式模式空间奥米克方法.
  • 为了在同一组织部分同时实现空间转录和多重蛋白质成像.
  • 为了生成单细胞解析的空间转录组地图,并探索生物通路.

主要方法:

  • DBiTplus将基于测序的空间转录学与多重蛋白质成像相结合.
  • 使用空间条形码和RNase H介导的cDNA检索来保存用于蛋白质分析的组织.
  • 使用计算管道来整合多omics数据和图像引导的解卷.

主要成果:

  • DBiTplus成功地在同一组织段上以单细胞分辨率映射了转录组和蛋白质组.
  • 与多种样本的相容性得到证明,包括具有挑战性的甲固定嵌入的临床标本.
  • 在人类淋巴瘤中发现了淋巴发育,进展和转变的机制.

结论:

  • DBiTplus是一个统一的工作流,用于空间分辨的单细胞图谱.
  • 能够在转录组尺度上对生物机制进行无偏见的细胞对细胞的探索.
  • 进步了解细胞异质性和在各种生物环境中的功能.