肠道微生物群和抗生素耐药性在多设施SPF小鼠中的比较元基因组学表征
Yujie Wang1, Caihong Wu1, Qi Zhu1
1Shanghai Laboratory Animal Research Center, Shanghai, 201203, China.
BMC microbiology
|January 16, 2026
概括
特定的无病原体小鼠肠道微生物组和抗生素耐药性基因 (ARG) 根据设施有很大差异. 繁殖环境对微生物群的影响比宿主菌株更大,影响ARG配置文件和抵抗机制.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 动物模型 动物模型
背景情况:
- 没有特定病原体 (SPF) 的小鼠是重要的临床前模型.
- 有限的高分辨率数据存在于SPF小鼠肠道微生物和抗生素耐药性基因 (ARG) 上.
研究的目的:
- 从多个设施的SPF小鼠中全面分析肠道微生物组和ARG.
- 调查繁殖环境与宿主菌株对微生物和抗体组合的影响.
主要方法:
- 从C57BL/6和BALB/cSPF小鼠中对五个设施的内含量的元基因组测序.
- 生物信息学分析包括分类学,功能和抗体组概况.
- 贝塔多样性分析以评估微生物社区结构.
主要成果:
- 确定了主导类型:巴西洛塔,巴克特罗伊多塔和阿克丁诺米塞托塔.
- 在各种设施中微生物群落和ARG配置文件的显著变化,繁殖环境比宿主菌株更有决定性.
- 主要的ARG与糖和四环素耐药性相关;在设施之间观察到不同的耐药性机制.
结论:
- 设施特定的环境显著塑造了SPF小鼠肠道微生物群和抵抗体.
- 提供了标准化临床前模型和评估微生物风险的基本数据.
- 强调需要在微生物组和ARG研究中考虑来源设施.
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