相关实验视频

Updated: Jun 27, 2026

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

野生和养殖的四大中国鱼的可追溯性使用基于高分辨率质谱的蛋白质学与多变量统计分析相结合

Xiaojing Yu¹, Jinyue Yang², Zhenxing Wu³

¹College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China.

Journal of agricultural and food chemistry

|January 16, 2026

概括

蛋白质学确定了24种蛋白质生物标志物,以区分野生和养殖的中国鱼. 这些发现有助于水产品的可追溯性和真实性,确保质量和商业价值.

科学领域:

水产产品的真实性认证
蛋白质组学是指蛋白质组学.
发现生物标志物的发现.

背景情况:

由于野生鱼和养殖鱼之间的质量和价值差异,水产品中可追溯性的需求日益增加.
需要强大的分析方法来区分鱼类的来源.

研究的目的:

开发和验证一种保护性战略,以区分野生和养殖的四大中国鱼.
为了识别标志着鱼类起源的蛋白质生物标志物.

主要方法:

利用了基于所有理论碎片离子质谱 (SWATH-MS) 的序列窗口获取蛋白质组学.
应用多变量统计分析,包括正交部分最小方形差异分析 (OPLS-DA) 和等级聚类分析 (HCA).

主要成果:

确定了24种蛋白质生物标志物,区分野生和养殖鱼种群.
通过OPLS-DA和HCA验证的生物标志物意义,显示出明显的丰度变化.
生物标志物参与能量代谢和结构发展.

结论:

与OPLS-DA结合的SWATH-MS蛋白质组学有效地歧视野生和养殖的中国鱼.
已识别的蛋白质生物标志物提供了对适应不同环境的分子见解.
OPLS-DA模型适用于水产产品的现实世界可追溯性.

关键词:

这就是SWATH-MS.四个主要的中国鱼.高分辨率质谱测量高分辨率质谱多变量统计分析多变量统计分析蛋白质组学蛋白质组学可以追溯的可追溯性.

更多相关视频

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

Published on: September 20, 2016

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

相关概念视频

FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...