Jove
Visualize
联系我们

相关概念视频

Next-generation Sequencing03:00

Next-generation Sequencing

97.8K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
97.8K
RNA-seq03:21

RNA-seq

11.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.8K
Complementary DNA01:44

Complementary DNA

31.3K
Overview
31.3K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

12.6K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
12.6K
Sanger Sequencing01:57

Sanger Sequencing

773.3K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
773.3K
Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

52.3K
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
52.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Spectral densities approximations of incidence-based locally treelike hypergraph matrices via the cavity method.

Physical review. E·2026
Same author

QTL mapping, breeding, and debugging Saccharomyces cerevisiae strains through Reiterated Mass Selection and backcrosSing (ReMaSSing).

Biotechnology for biofuels and bioproducts·2026
Same author

A combination of differential expression and network connectivity analyses identifies a common set of RNA splicing and processing genes altered with age across human tissues.

Aging·2025
Same author

Different patterns of association between maternal mental health and infant negative affect subdomains: Findings from the Germina cohort.

Infant behavior & development·2025
Same author

Inferences on the Watts-Strogatz Model: A Study on Brain Functional Connectivity.

Neuroinformatics·2025
Same author

Exclusive breastfeeding is associated with the gut microbiome maturation in infants according to delivery mode.

Gut microbes·2025
Same journal

From Pixels to Patterns: A Multidimensional Framework to Decode Cytoskeletal Organization.

Computational and structural biotechnology journal·2026
Same journal

A Large Concept Model for Mechanistic Simulation of Disease Trajectories: A Hypothesis-Generating Exemplar for Pediatric Acute Lymphoblastic Leukemia.

Computational and structural biotechnology journal·2026
Same journal

Adversarial Sequence Mutations in AlphaFold and ESMFold Reveal Nonphysical Structural Invariance, Confidence Failures, and Concerns for Protein Design.

Computational and structural biotechnology journal·2026
Same journal

High-Throughput Prediction of Protein-Protein Interactions Uncovers Hidden Molecular Networks in Biosynthetic Gene Clusters.

Computational and structural biotechnology journal·2026
Same journal

A Region-Aware Structured Framework Improves Prediction of Gene Expression from DNA Methylation.

Computational and structural biotechnology journal·2026
Same journal

Ensemble Machine Learning Approaches Predict Survival in Lower-Grade Glioma Based on Glycosphingolipid Gene Expression and Metabolic Modeling.

Computational and structural biotechnology journal·2026
查看所有相关文章
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jan 18, 2026

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

34.6K

潘塞翁-DNA:多功能编码解码系统,集成适应性NGS预处理算法,用于DNA数据存储.

Adriano Galindo Leal1, Thiago Yuji Aoyagi1, André Guilherme Costa-Martins1

  • 1Artificial Intelligence and Analytics Department, Institute for Technological Research, São Paulo, 05508-901, SP, Brazil.

Computational and structural biotechnology journal
|January 16, 2026
PubMed
概括
此摘要是机器生成的。

Pantheon-DNA是DNA数据存储的新管道,可以高效地处理大型数据集. 它实现了高数据可检索性,并使用新的方法来防止DNA序列编码和检索过程中的错误.

关键词:
DNA数据存储 DNA数据存储编码方案 编码方案序列数据预处理 序列数据预处理软件工程 软件工程 软件工程

更多相关视频

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

12.2K
A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
09:10

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

10.0K

相关实验视频

Last Updated: Jan 18, 2026

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

34.6K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

12.2K
A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
09:10

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

10.0K

科学领域:

  • 生物技术是生物技术.
  • 生物信息学是一种生物信息学.
  • 数据存储数据存储数据存储

背景情况:

  • DNA数据存储提供了高密度,但面临着可扩展性和错误挑战.
  • 重复的DNA序列可能会导致分子级别的错误,并使数据处理复杂化.
  • 从DNA中有效管理和检索大型数据集对于实际应用至关重要.

研究的目的:

  • 介绍Pantheon-DNA,一个端到端的处理管道用于DNA数据存储.
  • 解决DNA数据存储系统的可扩展性和效率挑战.
  • 提高基于DNA的数据存储中的数据可检索性和可靠性.

主要方法:

  • 开发了一个数据排列方案和随机化程序,以防止有问题的DNA序列模式.
  • 实现了块数据架构,以增强并行处理和检索.
  • 利用编码数据结构的先前知识来简化预处理和减少计算复杂性.

主要成果:

  • 在低错误率 (LER) 和高错误率 (HER) 条件下,在10倍覆盖率下实现≥99.996%的数据可检索性.
  • 在合成和测序实验中,成功编码和解码了1.59 MB的数据,其中包含多个文件.
  • 通过预处理管道演示了减少计算复杂性和简化集群程序.

结论:

  • 潘提昂-DNA有效地解决了DNA数据存储的可扩展性和效率.
  • 提出的方法提高了通过实验结果验证的数据可靠性和可检索性.
  • 未来的工作重点是改进错误纠正,特别是用于indel恢复,并优化预处理.