不受压力的细胞是相似的,但受压力的细胞是不同的:酵母菌应激反应中的环境和单细胞异质性
bioRxiv : the preprint server for biology
|January 16, 2026
概括
细胞应激反应在单个细胞之间有很大差异. 这项研究揭示了抑制的基因,而不是诱导的基因,最好识别受压力的细胞,突出了单细胞分析以了解细胞适应.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 细胞生物学 细胞生物学
背景情况:
- 细胞应激反应对生存和疾病至关重要,但人口平均值掩盖了个体细胞变异.
- 了解细胞异质性是充分理解应激适应和病理学的关键.
研究的目的:
- 用单细胞数据重新定义应激反应识别,超越平均信号.
- 调查可转移元素 (TE) 表达如何与压力期间编码基因表达有关.
- 发现稳定的分子特征,区分受压力和不受压力的细胞.
主要方法:
- 在各种压力下对大约13,000个*Saccharomyces cerevisiae*细胞进行高通量单细胞RNA测序.
- 将可转换元素 (TE) 与编码基因一起进行注释,以捕获超过6600个表达特征.
- 平均与单细胞表达模式的比较分析,以确定预测性压力标志物.
主要成果:
- 基于平均值的应激反应定义掩盖了显著的细胞异质性.
- 压力诱导的基因程序是特定条件的,并且是个体细胞压力的不良预测器.
- 压力抑制的程序,特别是与核糖体相关的基因,提供了一个稳定的,交叉数据集签名,用于识别受压力的细胞.
- 受到压力的细胞表现出不同的转录反应,其中一些激活了正规程序,而另一些则调高了TE的调节.
结论:
- 单细胞分辨率对于准确理解细胞应激反应至关重要,揭示了通过平均化掩盖的模式.
- 安娜·卡列尼娜原则适用,无压力细胞显示均的生长程序,而压力细胞则分离.
- 在单细胞水平上,TEs和正规压力程序显示了反向的关系,这表明细胞命运是相互排斥的.
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