在没有选择压力的情况下,Candida auris可以获得抗真菌耐药性
bioRxiv : the preprint server for biology
|January 16, 2026
概括
黄菌可以发展耐药性,并在实验室运输过程中改变其形状,即使没有抗真菌暴露. 这凸显了在真菌学实验室之间交换临床菌株时需要标准化的协议.
科学领域:
- 医学真菌学 医学真菌学
- 抗微生物耐药性 抗微生物耐药性
- 基因组学就是基因组学.
背景情况:
- 黄是一种重要的人类真菌病原体,引起高死亡率的疫情.
- 治疗失败与抗真菌药物耐药性和菌株变异性有关.
- 种内变异性和诱导的超突变有助于致病性和治疗失败.
研究的目的:
- 为了调查Candida auris在实验室间菌株交换期间的遗传和表型变化.
- 为了识别与药物耐药性和形态发生变化相关的突变.
- 评估纸运输条件对C. auris遗传稳定性的影响.
主要方法:
- 使用CDC mycoSNPs管道进行全基因组测序.
- 按照临床和实验室标准研究所 (CLSI) 的指导方针进行抗真菌敏感性测试.
- 模拟过纸运输条件的实验,以诱导遗传和表观遗传变化.
主要成果:
- 在过纸上进行C. auris的实验室间交换导致了不同的形态型的出现.
- 这些形态型显示出药物耐药性和形态发生的变化,与阿醇和乙康丁耐药基因 (TAC1B,MRR1,FKS2) 的突变有关.
- 在模拟运输过程中发生了遗传和表观遗传变化,解释了形态遗传切换和改变的醇耐药性.
结论:
- 黄菌可以获得影响药物耐药性的突变,而无需抗真菌暴露.
- 常规的菌株处理和交换可以导致显著的遗传和表型多样化.
- 交换临床C. auris菌株的标准化协议对于可靠的研究和诊断至关重要.
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