肌肉中间歇性AP-1激活有助于运动诱导的健康益处
bioRxiv : the preprint server for biology
|January 16, 2026
概括
定期运动通过分子通路改善健康. 这项研究确定AP-1转录因子,特别是cFos/JunD,对于运动诱导的适应至关重要,它将间歇性训练与增强的肌肉功能和新陈代谢联系起来.
科学领域:
- 分子生物学分子生物学
- 运动生理学 运动生理学
- 衰老研究研究 衰老研究
背景情况:
- 众所周知,定期体育炼可以延长健康寿命,但与运动和持久益处相关的精确分子机制尚未完全理解.
- 增强器RNAs (eRNAs) 作为增强器活动的敏感指标,提供对转录调节的见解.
研究的目的:
- 阐明运动诱导骨肌肉有益适应的分子机制.
- 确定关键的转录因子和参与运动诱导的转录组变化的信号通路.
- 调查运动间歇性在调解这些适应中的作用.
主要方法:
- 全球核运行测序 (GRO-seq) 用于对跑步机运行后小鼠骨肌中的eRNA和增强剂活性进行分析.
- 在收缩C2C12神经管的选方法中,确定了特定的转录因子.
- 在小鼠中进行了主导负cFos突变 (A-Fos) 的肌肉特异性过度表达和cFos/JunD的间歇性/连续性过度表达.
主要成果:
- 激活蛋白-1 (AP-1) 转录因子,特别是cFos/JunD,被确定为运动激活增强剂的关键调节者.
- 破坏cFos功能减弱运动诱导的转录变化和肌肉功能受损.
- 间歇性过度表达cFos/JunD模仿了运动效应,增强了线粒体密度,肌肉力量,耐疲劳性和耐葡萄糖性.
结论:
- 一个涉及AP-1的转录调节通路,由运动间歇性驱动,将收缩应激与有益的适应联系起来.
- 培训中的恢复周期对于调解这些积极影响至关重要.
- 这些发现为AP-1在衰老和健康中的复杂作用提供了洞察力,强调了压力因素时间的重要性.
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