Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

23.8K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
23.8K
RNA-seq03:21

RNA-seq

11.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Emergence of fibrotic pericytes and their transcriptional regulation in pulmonary fibrosis.

Nature communications·2026
Same author

AlveolEye: rapid and precise lung morphometry guided by computer vision.

American journal of physiology. Lung cellular and molecular physiology·2026
Same author

Identification of a conserved sequence of disease progression in Idiopathic Pulmonary Fibrosis.

American journal of respiratory and critical care medicine·2026
Same author

FOXF2 regulates pericyte-endothelial signaling required for vascular homeostasis after neonatal hyperoxic lung injury.

Nature communications·2026
Same author

Optimized primary organotypic culture from murine neonatal tracheal airway epithelial cells.

American journal of physiology. Lung cellular and molecular physiology·2026
Same author

Aberrant cellular communities underlying disease heterogeneity in chronic obstructive pulmonary disease.

Nature genetics·2026
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
查看所有相关文章

相关实验视频

Updated: Jan 18, 2026

Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis
07:40

Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis

Published on: May 16, 2025

908

用两条线捕鱼:一种混合方法来实现空间转录基因的发现

Arianna L Williams-Katek, Saahithi Mallapragada, Evan D Mee

    bioRxiv : the preprint server for biology
    |January 16, 2026
    PubMed
    概括
    此摘要是机器生成的。

    这项研究引入了一种双化学空间转录学方法,结合了高灵敏度和广泛覆盖面板. 这种方法增强了细胞保留和数据丰富性,用于敏感的基因分析和发现.

    更多相关视频

    Robust 3D DNA FISH Using Directly Labeled Probes
    12:16

    Robust 3D DNA FISH Using Directly Labeled Probes

    Published on: August 15, 2013

    35.4K
    Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
    06:26

    Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

    Published on: September 20, 2020

    3.5K

    相关实验视频

    Last Updated: Jan 18, 2026

    Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis
    07:40

    Spatially Compact Arrangement of Larval Zebrafish Sections for Spatial Transcriptomic Analysis

    Published on: May 16, 2025

    908
    Robust 3D DNA FISH Using Directly Labeled Probes
    12:16

    Robust 3D DNA FISH Using Directly Labeled Probes

    Published on: August 15, 2013

    35.4K
    Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
    06:26

    Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

    Published on: September 20, 2020

    3.5K

    科学领域:

    • 分子生物学分子生物学
    • 基因组学就是基因组学.
    • 生物技术是生物技术.

    背景情况:

    • 空间转录学使得在组织背景下进行基因表达分析.
    • 目前的方法面临着分析基因数量和每基因敏感性之间的权衡.
    • 高灵敏度面板测试的基因较少,而广泛覆盖面板的灵敏度较低.

    研究的目的:

    • 为空间转录组学开发一种新的双化学方法.
    • 在单个组织部分上结合高灵敏度 (Xenium V1面板) 和广泛覆盖 (Prime 5K面板).
    • 克服现有的空间转录学方法的局限性.

    主要方法:

    • 通过联合混合Prime和Xenium V1探测器开发了一种"双化学"方法.
    • 在同一块组织上顺序执行V1和Prime解码化学物质.
    • 在人类肺组织微阵列上验证了该方法.

    主要成果:

    • 在独立的V1和Prime化学运行和双化学方法之间实现了高一致性.
    • 在双重运行中观察到重叠基因的类似表达模式,证实了试验准确性.
    • 证明了高灵敏度和广泛覆盖的概况的成功整合.

    结论:

    • 双化学方法有效地整合了高灵敏度和广泛覆盖的空间转录组学.
    • 这种方法增强了细胞的保留,并提供了更丰富的生物信息.
    • 能够进行敏感基因表达分析以及在空间转录组学研究中进行更广泛的发现.