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高时间分辨率成像单个免疫细胞迁移在vivo通过超亮NIR-II有机探针

Ying Chen^1,2, Yang Yang³, Baofeng Yun^1,2

¹Department of Chemistry, College of Smart Materials and Future Energy, New Cornerstone Science Laboratory, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials and Ichem, Fudan University, Shanghai, P. R. China.

Advanced materials (Deerfield Beach, Fla.)

|January 16, 2026

概括

研究人员开发了新的有机探针,用于单个免疫细胞的高速,非侵入性体内成像,从而可以实时跟踪中性粒细胞迁移和中风模型中的细胞相互作用.

科学领域:

生物医学成像技术生物医学成像技术
免疫学免疫学免疫学
神经科学是一个神经科学.

背景情况:

免疫细胞迁移在各种疾病中至关重要.
实时在体内追踪运动性免疫细胞仍然是一个挑战.
了解细胞相互作用是治疗开发的关键.

研究的目的:

开发先进的成像探测器,用于非侵入性,高速的体内免疫细胞跟踪.
在疾病模型中实现免疫细胞迁移和相互作用的实时分析.
促进对免疫反应中的细胞动态的基础研究.

主要方法:

引入在第二近红外 (NIR-II) 窗口 (1000-1700 nm) 中运行的高亮度有机探针.
开发具有高亮度 (>5000 M−1 cm−1) 的探测器 (DCPD).
探针的应用用于非侵入性,高速 (高达~50 fps) 的单个免疫细胞成像 in vivo.

主要成果:

证明了小鼠大脑中单个免疫细胞的非侵入性,高速成像.
在中风模型中成功分析了中性粒细胞迁移模式.
观察了中性粒细胞和注入的介质干细胞之间的实时相互作用.

结论:

开发的NIR-II探针使得在体内免疫细胞成像方面具有前所未有的时间分辨率.
这项技术有助于研究神经疾病中的动态细胞过程.
进步为研究免疫反应和疾病发病的单细胞动态铺平了道路.

关键词:

在NIR-II光成像中使用光成像.免疫细胞迁移的迁移一个单细胞追踪系统.超明亮的有机探测器视频速率成像成像技术

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