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Updated: Jan 18, 2026

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
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Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Published on: February 5, 2019

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为蛋白质,RNA和等离子体工程进行无和高效的位点导向突变发生

Xiang-Jiao Yang1,2,3,4

  • 1Rosalind and Morris Goodman Cancer Institute, McGill University, Montreal, Quebec, Canada.

Current protocols
|January 16, 2026
PubMed
概括

这项研究引入了一种高效的局部定向突变发生法,使用PCR与3'-overhang原料,实现近100%的效率. 这种技术使得无的磁带突变发生在蛋白质和等离子体工程中实现DNA片段的删除,插入或替换.

关键词:
这是一个PCRPCR.表观遗传学是指表观遗传学.基因组编辑 基因组编辑突变发生的突变发生.病毒 病毒 病毒 病毒

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Conservative Site-specific Recombination and Phase Variation02:53

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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