一个活性物质模型捕捉了Drosophila形态发生过程中幼虫上皮细胞中actomyosin振荡的空间动态
Euan D Mackay1, Aimee Bebbington2,3,4, Jens Januschke1
1Division of Computational Biology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom.
概括
细胞形状和极性显著影响介性actomyosin网络动态,在Drosophila发育过程中驱动脉冲收缩. 这些收缩是actomyosin网络本身的一个新兴特性.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 发展生物学 发展生物学
背景情况:
- 细胞中介性actomyosin网络产生细胞机械力,并在形态发生过程中表现出振荡行为.
- 果幼虫上皮细胞 (LECs) 中的脉冲性收缩先于被组织细胞所取代,但它们的控制机制尚不清楚.
研究的目的:
- 为了在体内研究LECactomyosin网络的时空动态.
- 了解细胞几何,极性和actomyosin组织在收缩行为中的作用.
主要方法:
- 结合体内4D显微镜与使用活性弹性体模型的数值模拟.
- 将模型应用于现实的细胞几何形状和边界条件,由细胞极性告知.
主要成果:
- 活性弹性体模型准确地复制了活体中对actomyosin动态的观察结果.
- 使用现实的细胞几何形状的模拟与实验数据的一致性比使用矩形域的模拟更好.
- 细胞形状,极性和actomyosin组织被发现可以编码确定网络动态.
结论:
- 细胞几何学和极性信息的边界条件对于理解中介性actomyosin网络动态至关重要.
- 阿克托米奥辛网络的脉冲振荡行为是一种新兴的属性,不是由上游信号驱动的.
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