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相关概念视频

Gas Chromatography–Mass Spectrometry (GC–MS)01:14

Gas Chromatography–Mass Spectrometry (GC–MS)

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Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
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Gas Chromatography: Introduction01:13

Gas Chromatography: Introduction

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Gas chromatography (GC) is a technique for separating and analyzing volatile compounds in a sample. Its primary purpose is to identify and quantify components in complex mixtures, making it essential in fields such as environmental analysis, pharmaceuticals, and petrochemicals. GC is also called vapor-phase chromatography (VPC) or gas-liquid partition chromatography (GLPC).
In GC,  a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a...
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Mass Spectrometry: Complex Analysis01:21

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Sample Preparation for Analysis: Overview01:21

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Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
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使用便携式GC-MS进行尿液VOC分析的固体相微提取:与基板仪器仪表进行方法开发和验证.

Mark Woollam1, Serenidy Eckerle2, Eray Schulz1

  • 1Integrated Nanosystems Development Institute, Indiana University Indianapolis, Indianapolis, IN, 46202, USA; Department of Chemistry & Chemical Biology, Indiana University Indianapolis, Indianapolis, IN, 46202, USA.

Talanta
|January 16, 2026
PubMed
概括

这项研究开发了一种新的固体相微提取方法,用于便携式气色谱-质谱检测尿液挥发性有机化合物 (VOC). 这种新方法增强了对潜在的护理点疾病诊断的VOC检测.

关键词:
化学测量分析的化学分析.气色谱 - 质谱学 (GC-MS) 气色谱便携式系统 便携式系统固体相微提取 (SPME) 是一种微提取技术.挥发性有机化合物 (VOC) 是一种挥发性有机化合物.

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科学领域:

  • 分析化学 分析化学
  • 生物标志物发现发现
  • 临床诊断 临床诊断 临床诊断

背景情况:

  • 尿液中的生物挥发性有机化合物 (VOC) 显示出作为非侵入性疾病生物标志物的前景.
  • 气色谱-质谱 (GC-MS) 是VOC分析的标准,但通常需要大型的实验室设备.
  • 便携式GC-MS为护理点测试提供了潜力,但对于生物样本,方法需要优化.

研究的目的:

  • 开发和优化固体相微提取 (SPME) 方法与便携式GC-MS相结合,用于检测尿液头部空间中的生物VOC.
  • 为了提高尿液VOC分析的灵敏度,可重复性和色谱分辨率.
  • 为了验证便携式GC-MS方法与标准的GC-MS系统对比.

主要方法:

  • 优化样品准备,列上和MS参数,用于SPME便携式GC-MS.
  • 将SPME与标准空气探针采样方法进行比较.
  • 使用UTAK尿液标准评估日内和日间的重复性.
  • 来自健康志愿者的尿样分析,并通过基准GC-MS进行确认.

主要成果:

  • 与空气探测器相比,SPME方法显著丰富了VOC (平均log2倍变化为3.5).
  • 实现了良好的重复性,VOCs通常显示相对标准偏差 (RSD) 低于25%.
  • 便携式GC-MS系统成功地在健康志愿者中区分了VOC档案,结果被基板GC-MS证实.

结论:

  • 一种新的SPME便携式GC-MS方法是有效的分析尿液VOCs.
  • 这种方法表明了敏感,可重复和准确的VOC分析的潜力.
  • 经过验证的便携式平台为未来的临床诊断应用提供了信心.