策略依赖的阿纳莫克斯对硫法的耐受性:从EPS防御到微生物网络合作的级联
Yunjing Wang1, Cong Wang1, Qing Ye1
1National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China.
Journal of hazardous materials
|January 16, 2026
概括
重复暴露于硫法迪亚会伤害阿纳莫克斯细菌,但逐渐增加的度会保护它们的去除. 细胞外聚合物中紧密结合的蛋白质保护关键酶免受抗生素的压力.
科学领域:
- 环境微生物学环境微生物学
- 污水处理 污水处理 污水处理
- 生物技术是生物技术.
背景情况:
- 亚纳莫克斯过程对于去除至关重要,但易受抗生素抑制的影响.
- 了解anammox对硫黄 (SDZ) 暴露策略的反应对于系统稳定性至关重要.
研究的目的:
- 为了比较反复与提高SDZ暴露策略在anammox系统的稳定性和弹性.
- 阐明SDZ抑制和anammox防御的分子机制.
主要方法:
- 两种SDZ暴露策略的比较分析 (重复与增加度).
- 评估特定的anammox活动 (SAA) 和总去除效率 (TNRE).
- 对细胞外聚合物物质 (EPS) 组成,酶功能和分子对接模拟的分析.
主要成果:
- 重复的SDZ暴露 (R0-R3) 导致累积抑制,减少SAA和TNRE.
- 提高SDZ度 (R4) 保持高TNRE (>85%) 即使在50mg/LSDZ.
- 在EPS中紧密结合的蛋白质 (TB-PN) 屏蔽了SDZ中的酸盐减少酶 (NIR) 等关键酶,从而保留了R4.4中的功能.
结论:
- 提高SDZ度策略与重复暴露相比,提高了anammox的弹性.
- 在EPS中的紧密结合蛋白 (TB-PN) 对于anammox系统中抗生素应激的多级防御至关重要.
- 结果为优化抗生素污染环境中的anammox稳定性提供了洞察力.
相关概念视频
Stringent Response in E. coli
300
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
300
Gene Regulation in Microbial Communities: Quorum Sensing
533
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
533
Sulfur Assimilation
321
Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to...
321
Development of Antibiotic Resistance
1.3K
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
1.3K
Transduction
1.3K
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
1.3K
Combined Effects of Drugs: Synergism
6.8K
Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...
Such synergistic combinations...
6.8K


