微生物通过Aspergillus niger将红素转化为酸
Jiayuan Jia1, Ziyu Dai1, Yinglei Han2
1Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA 94608, USA; Pacific Northwest National Laboratory, 902 Battelle Boulevard, Richland, WA 99354, USA.
Bioresource technology
|January 16, 2026
概括
在这项研究中,Aspergillus niger被设计成将红素转化为有价值的工业化学品酸. 修改后的真菌有效地处理了复杂的素流,证明了可持续生物转换的新途径.
科学领域:
- 生物技术是生物技术.
- 生物化学 生物化学
- 微生物工程 微生物工程
背景情况:
- 素是主要的可再生芳香资源,由于其复杂的结构,在生物转化方面存在挑战.
- 开发高效的素增值方法对于可持续的化学品生产至关重要.
研究的目的:
- 为了设计Aspergillus niger用于生物转化素衍生芳香物和基催化脱聚合 (BCD) 素到酸.
- 优化生产条件并评估工程菌株在利用真实素流中的能力.
主要方法:
- 亚斯伯吉路斯尼格尔的基因工程,包括过度表达C4二碳酸盐输送物C4T318.
- 在缓冲条件下进行中等优化和栽培.
- 评估特定的素衍生物芳香物的同化和BCD素液的转化.
主要成果:
- 经过工程改造的A. niger菌株从具有代表性的素衍生芳香物,如4-基酸和p-酸中产生高达3.9g/L的酸.
- 从树和土中直接转化BCD木质素液,得到高达0.82g/L的酸.
- 通过输送器过度表达和媒介优化,通过输送器过度表达和媒介优化实现了增强的酸分泌和生产.
结论:
- 尼日尔菌可以被设计成一个可行的平台,用于直接的真菌转化真实木质素流成酸.
- 这项研究为可持续地将红素价值化为高价值的C4二氧酸建立了一个有前途的途径.
- 该研究强调了微生物生物转化在利用复杂,异构的芳香原料方面的潜力.
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