连续流量纸喷雾电离质谱测量用于组织中抗癌药物的深入表征:解决质谱复杂性的问题
Pallab Basuri1, Konrad Klinghammer2, Oliver Klein3
1Department of Bioanalytical Chemistry, Institute of Chemistry, Humboldt-Universität zu Berlin, 12489 Berlin, Germany.
Journal of the American Society for Mass Spectrometry
|January 16, 2026
概括
连续流纸喷雾电离质谱仪 (CFPSI MS) 能够快速检测组织中的抗癌药物. 这种方法量化了药物吸收,palbociclib在小鼠模型中显示了最高的生物吸收.
科学领域:
- 分析化学 分析化学
- 质谱测量质量谱测量
- 药理学 药理学是指药理学的学科.
背景情况:
- 在固体组织中精确量化抗癌药物对于治疗药物监测至关重要.
- 现有的组织药物分析方法可能是耗时和复杂的.
- 了解药物在组织中的分布和吸收对于优化治疗疗效至关重要.
研究的目的:
- 引入和验证一种新的连续流纸喷雾电离质谱法 (CFPSI MS) 方法.
- 为了能够在固体组织样本中快速检测和半定量分析抗癌药物.
- 在临床前模型中研究特定抗癌药物的差异性吸收和来源化学反应.
主要方法:
- 开发了一种连续流动的纸张喷雾电离技术 (CFPSI).
- 在患者衍生异种移植 (PDX) 鼠标组织样本中分析palbociclib,copanlisib和olaparib的CFPSI MS的应用.
- 使用双重质谱测量来探索药物反应性和光谱复杂性.
主要成果:
- 在固体组织样本中,CFPSI MS成功检测和表征了抗癌药物.
- 观察到Palbociclib,Copanlisib和Olaparib的吸收差异,Palbociclib表现出最高的生物吸收.
- 双重质谱检测显示了显著的光谱复杂性,这是由于药物的来源化学反应性.
结论:
- CFPSI MS是用于组织中抗癌药物的快速,半定量分析的一个有前途的技术.
- 这项研究强调了光谱解释在复杂的生物矩阵中对于准确的药物定量的重要性.
- 这些发现支持进一步开发CFPSI MS,用于治疗药物监测中的临床应用.
关键词:
这是CFPSI.分析 分析 分析这是一种抗癌药物.这是一个连续的连续的连续.药物 药物 药物 药物 药物 药物流动 流动 流动 流动质量质量质量质量质量质量质量.纸张 纸张 纸张 纸张快速的 快速的 快速的样品. 样品. 这些样品.这是光谱的光谱.组织组织组织组织组织.更多相关视频
09:00A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
Published on: April 18, 2025
1.2K
07:55Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
Published on: June 21, 2019
6.1K
相关概念视频
01:21Tandem Mass Spectrometry
2.3K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.3K
01:21Mass Spectrometry: Complex Analysis
1.6K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.6K
01:19MALDI-TOF Mass Spectrometry
6.5K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
6.5K
01:19Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
1.8K
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
1.8K
01:12Electrospray Ionization (ESI) Mass Spectrometry
2.1K
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
2.1K
01:19Mass Spectrometry: Overview
8.2K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
8.2K