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多层宿主工程的Saccharomyces cerevisiae增强板球麻病毒 (CrPV) 内部核糖体入口部位介导翻译

Zeyu Cao¹, Xiaotong Zou¹, Koichi Ito¹

¹Department of Computational Biology and Medical Science, Graduate school of Frontier Sciences, The University of Tokyo, Kashiwa-shi, Chiba 277-8562, Japan.

ACS synthetic biology

|January 17, 2026

概括

工程酵母宿主因素显著提升了麻病毒 (CrPV) 内核糖核体进入点 (IRES) 的翻译. 这一策略增强了合成生物学应用的非正典翻译.

科学领域:

分子生物学分子生物学
合成生物学合成生物学
遗传学遗传学是一个

背景情况:

内部核糖体进入点 (IRES) 是RNA元素,可以实现独立于的翻译.
麻痺病毒 (CrPV) IRES在Saccharomyces cerevisiae中的活性有限,阻碍了其在合成生物学中的使用.
提高IRES活动需要理解和操纵主机翻译机器.

研究的目的:

开发一种酵母工程策略,以加强CRPV的IRES介导的翻译.
通过基于报告者的屏幕来识别影响IRES活动的宿主因素.
在酵母中建立工程非正典翻译的框架.

主要方法:

利用基于报告者的屏幕来识别影响IRES活动的主机因素.
实施了针对翻译启动,tRNA修改和mRNA稳定性的宿主修改.
结合无意义介导的衰变缺陷,tad3温度敏感的等位基因和eIF4E过度表达.

主要成果:

确定了IRES刺激与基本上限依赖转换之间的权衡.
在工程酵母菌株中实现了高达十倍的记者输出.
通过多层次的主机工程,成功增强了CRPV IRES活动.

结论:

主机工程可以显著改善由病毒IRES元素介导的非正典翻译.
针对翻译启动,tRNA修饰和mRNA稳定性的多方方法是有效的.
这项工作为工程酵母在合成生物学中增强IRES功能提供了原则证明.

关键词:

板球麻病毒IRES,就是板球麻病毒.翻译控制翻译控制酵母工程是一种酵母工程.在mRNA的稳定性上.在tRNA修改过程中.

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