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工程节能Saccharomyces cerevisiae用于甲醇和CO2的同化

Wei Zhong^1,2, Nana Liu^1,2, Binbin Chen^1,2

¹Center of Synthetic Biology and integrated Bioengineering, Westlake University, Hangzhou, Zhejiang, China.

Nature communications

|January 17, 2026

概括

工程酵母有效地将甲醇转化为能量 (ATP和NADH) 和生物质. 这种强大的平台可实现二氧化碳的吸收,推进C1生物转化技术.

科学领域:

生物技术是生物技术.
合成生物学合成生物学
微生物工程微生物工程

背景情况:

甲醇是微生物生物转化的主要C1原料.
工程化Saccharomyces cerevisiae在甲醇同化过程中经常经历能量限制.

研究的目的:

开发一种高能效的甲基型Saccharomyces cerevisiae菌株,以提高甲醇利用率.
在甲醇代谢过程中增强ATP和NADH生成.
为了使甲醇衍生中间体和二氧化碳的同同化.

主要方法:

异质甲醇-甲-甲酸盐 (MFF) 氧化路径的工程.
适应性实验室进化Saccharomyces cerevisiae的. 这是一个很好的例子.
关键能量模块 (Fdh1sc, Adh2m, Aoxm, Rgi2m) 的表征.
研究甲诱导的DNA损伤.

主要成果:

开发了SC-AOX25,一种高能效的甲基型S. cerevisiae菌株.
在甲醇代谢过程中,SC-AOX25有效地产生ATP和NADH.
甲醇中间体和二氧化碳通过原生和非原生途径 (卡尔文循环) 的同同同化.
基于DNA损伤分析,确定了甲醇排毒和表型增强的策略.

结论:

SC-AOX25是用于C1工程的强大且节能的甲基变平台.
这种工程菌株在甲醇发酵过程中促进了二氧化碳的同化.
这项工作推进了利用甲醇作为原料的微生物生物转化潜力.

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