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相关概念视频

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis09:56

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

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This protocol demonstrates how to use the Auto-CHO software for hierarchical and programmable one-pot synthesis of oligosaccharides. It also describes the general procedure for RRV determination experiments and one-pot glycosylation of...
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Oligosaccharide Assembly01:24

Oligosaccharide Assembly

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Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
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Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors10:17

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors

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We describe here the high-throughput detection and quantification of fucosylated human milk oligosaccharides (HMOs) using a whole-cell biosensor. We also demonstrate here, the adaptation of this platform towards analysis of HMO production strains, focusing on improving the signal to noise...
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Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages08:46

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For this study synchrotron radiation micro-tomography, a non-destructive three-dimensional imaging technique, is employed to investigate an entire microelectronic package with a cross-sectional area of 16 x 16 mm. Due to the synchrotron's high flux and brightness the sample was imaged in just 3 min with an 8.7 µm spatial...
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Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications14:43

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We present a method for rapid, reversible immobilization of small molecules and functionalized nanoparticle assemblies for Surface Plasmon Resonance (SPR) studies, using sequential on-chip bioorthogonal cycloaddition chemistry and antibody-antigen...
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Anastasis is technically challenging to detect in vivo because the cells that have reversed the cell death process can be morphologically indistinguishable from normal healthy cells. Here we describe protocols for detecting and tracking cells that undergo anastasis in live animals by using our newly developed in vivo CaspaseTracker biosensor...
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相关实验视频

Updated: Jan 20, 2026

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
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Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

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LDOB:可编程的多维乳糖衍生寡糖生物传感器.

Li-Hua Liu1, Bo Xu2, Ying Huang3

  • 1School of Basic Medical Sciences, Hubei University of Science and Technology, Xianning, 437100, PR China; Bio-Fermentation Research Center, Xiamen Yuanzhidao Biotechnology Co., Ltd., EYOSON Group Co., Ltd., Xiamen, 361028, PR China; Guangdong Provincial Key Laboratory of Marine Biotechnology, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, STU-UNIVPM Joint Algal Research Center, College of Science, Shantou University, Shantou, 515063, Guangdong, PR China.

Biosensors & bioelectronics
|January 18, 2026
PubMed
概括

研究人员开发了乳糖衍生的寡糖生物传感器 (LDOB),用于实时监测母乳寡糖 (HMO) 产量. 该工具加速了用于生物制造的高产量HMO生产微生物菌株的选.

关键词:
生物传感器是一种生物传感器.高通量选的高通量选人类牛奶中的寡糖类.乳糖是一种乳糖.这就是de novo合成.

更多相关视频

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors
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Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
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相关实验视频

Last Updated: Jan 20, 2026

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09:56

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Published on: September 6, 2019

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Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors
10:17

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Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
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科学领域:

  • 生物技术是生物技术.
  • 微生物工程 微生物工程
  • 合成生物学 合成生物学

背景情况:

  • 人类牛奶寡糖 (HMO) 具有重要的健康益处,但由于缺乏有效的分析工具,通过微生物合成进行可扩展的生产受到阻碍.
  • 对HMO生产的菌株改进需要快速和普遍的方法来评估生物合成效率.

研究的目的:

  • 开发一种全细胞生物传感器系统,用于实时监测和高通量查产生HMO的微生物菌株.
  • 用乳糖衍生的寡糖生物传感器 (LDOB) 设计大肠杆菌以实现高效的菌株选择.

主要方法:

  • 通过基因工程构建表达乳糖衍生寡糖类生物传感器 (LDOB) 的大肠杆菌菌株.
  • 在LDOB传感电路中集成多维负反模块 (多压抑,向蛋白质降解,oRBS).
  • 验证LDOB信号与HMO产量 (2'-FL,LNnT) 的相关性,并与光激活滴滴分类 (FADS) 进行高通量选的合.

主要成果:

  • LDOB系统将乳糖消耗转化为反相关的生物质和光读数,从而实现实时HMO合成监测.
  • LDOB信号显示与关键HMO如2'-糖乳糖 (2'-FL) 和乳糖-N-新糖 (LNnT) 的产量有很强的一致性.
  • 使用LDOB和FADS的高通量查导致了显著的标位增强:2'-FL的42.8%和LNnT的86.4%.

结论:

  • LDOB为监测HMO合成和选高产产品菌株提供了卓越的工具,克服了微生物生物制造中以前的瓶.
  • 这种工程化生物传感器系统通过实现高效的菌株改进和加快生产流程,大大推进了HMO生物制造领域.