相关实验视频

Updated: Jan 20, 2026

Detection of Targetable Alterations in Non-small Cell Lung Cancer using Next-generation Sequencing

Detection of Targetable Alterations in Non-small Cell Lung Cancer using Next-generation Sequencing

Published on: October 10, 2025

通过布鲁萨托尔向FOXM1/PSAT1轴,可以抑制肺癌恶性进展

Siyi Ou¹, Xiaobo Wang², Yulan Sun²

¹Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, China; Department of Respiratory and Critical Care Medicine, the First People's Hospital of Qinzhou, Qinzhou, Guangxi 535000, China.

Pathology, research and practice

|January 18, 2026

概括

布鲁萨托尔是一种来自Brucea javanica的化合物,通过降低索胺转移酶1 (PSAT1) 的调节来抑制肺癌. FOXM1/PSAT1通路是布鲁萨托尔的关键.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
药理学药理学是指药理学的学科.

背景情况:

布鲁萨托尔 (BRU),来自Brucea javanica,表现出抗癌性质.
布鲁萨托尔在肺癌中的确切作用机制尚不完全理解.
确定下游目标对于了解布鲁萨托尔的治疗潜力至关重要.

研究的目的:

阐明布鲁萨托尔在肺癌中的下游目标和机制.
调查素氨基转移酶1 (PSAT1) 在布鲁萨托尔抗癌作用中的作用.
探索转录因子FOXM1在调节PSAT1.1中的参与.

主要方法:

定量实时PCR和西式斑点测试,以评估mRNA和蛋白质水平.
在体外和体内实验中评估PSAT1过度表达对布鲁萨托尔疗效的影响.
染色体免疫沉测试以确定FOXM1与PSAT1促进体的结合.

主要成果:

布鲁萨托尔显著降低了PSAT1.1的mRNA和蛋白质表达.
过度表达PSAT1可以抵消布鲁萨托尔在肺癌细胞中的抗瘤作用.
FOXM1直接与PSAT1促进体结合,增强其转录.
FOXM1上调取消了Brusatol对PSAT1的抑制作用,并促进了瘤细胞的存活.

结论:

FOXM1/PSAT1轴被确定为Brusatol在肺癌中的抗癌活性的关键调解器.
布鲁萨托尔通过向FOXM1/PSAT1通路,显示出作为肺癌新型治疗策略的潜力.
对这种途径的进一步研究可能会导致改善肺癌治疗方法.

关键词:

布鲁萨托尔 (Brusatol) 是一种类型的药物.在FOXM1中,肺癌是一种肺癌.在PSAT1中,我们使用了PSAT1.

更多相关视频

Anticancer Efficacy of Photodynamic Therapy with Lung Cancer-Targeted Nanoparticles

Anticancer Efficacy of Photodynamic Therapy with Lung Cancer-Targeted Nanoparticles

Published on: December 1, 2016

Detection of Lung Tumor Progression in Mice by Ultrasound Imaging

Detection of Lung Tumor Progression in Mice by Ultrasound Imaging

Published on: February 27, 2020

相关概念视频

Detection of Targetable Alterations in Non-small Cell Lung Cancer using Next-generation Sequencing

Detection of Targetable Alterations in Non-small Cell Lung Cancer using Next-generation Sequencing

This protocol describes an automated, ISO15189-accredited next-generation sequencing workflow for detecting targetable genomic alterations in non-small cell lung cancer (NSCLC) formalin-fixed paraffin-embedded...

Anticancer Efficacy of Photodynamic Therapy with Lung Cancer-Targeted Nanoparticles

Anticancer Efficacy of Photodynamic Therapy with Lung Cancer-Targeted Nanoparticles

Photodynamic therapy (PDT) is an alternative choice for lung cancer treatment. To increase the therapeutic effect of PDT, lung cancer-targeted nanoparticles combined with chemotherapy were developed. Both in vitro and in vivo anticancer efficacies of PDT with prepared nanoparticles were...

Detection of Lung Tumor Progression in Mice by Ultrasound Imaging

Detection of Lung Tumor Progression in Mice by Ultrasound Imaging

This protocol describes the steps taken to induce KRAS lung tumors in mice as well as the quantification of formed tumors by ultrasound imaging. Small tumors are visualized in early timepoints as B-lines. At later timepoints, relative tumor volume measurements are achieved by the measurement tool in the ultrasound...

Construction of An Orthotopic Xenograft Model of Non-Small Cell Lung Cancer Mimicking Disease Progression and Predicting Drug Activities

Construction of An Orthotopic Xenograft Model of Non-Small Cell Lung Cancer Mimicking Disease Progression and Predicting Drug Activities

This study presents an orthotopic non-small cell lung cancer (NSCLC) model based on intrapulmonary inoculation of multicellular spheroids of fluorescent A549-iRFP cells. The model recapitulates clinical NSCLC stages and responds to cisplatin, according to dynamic in vivo monitoring of long-wavelength fluorescence.

Multidimensional Coculture System to Model Lung Squamous Carcinoma Progression

Multidimensional Coculture System to Model Lung Squamous Carcinoma Progression

An in vitro model system was developed to capture tissue architectural changes during lung squamous carcinoma (LUSC) progression in a 3-dimensional (3D) co-culture with cancer-associated fibroblasts (CAFs). This organoid system provides a unique platform to investigate the roles of diverse tumor cell-intrinsic and extrinsic changes that modulate the tumor...

3D Co-culture of Lung Cancer Cells with CAFs: An In Vitro Model System to Study Tumor Progression

3D Co-culture of Lung Cancer Cells with CAFs: An In Vitro Model System to Study Tumor Progression

This video describes the two invitro model systems to capture the tissue architectural changes during lung squamous carcinoma progression in a 3-dimensional (3D) co-culture with cancer-associated fibroblasts. This system provides a unique platform to investigate the role of intrinsic and extrinsic changes that affect the tumor...