相关实验视频

Updated: Jan 20, 2026

A Data-Driven Approach to Quantifying Immune States in Sepsis

A Data-Driven Approach to Quantifying Immune States in Sepsis

Published on: February 7, 2025

东方方药通过调节巨细胞糖解和败血症中的免疫功能来缓解严重的炎症

Yanjun Zheng¹, Li Chen², Hongqi Li²

¹Shanghai Key Laboratory of Molecular Imaging, Zhoupu Hospital, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China.

Free radical biology & medicine

|January 18, 2026

概括

东方 (Ori) 向糖解酶PFKL,以减少败血症中的炎症和代谢功能障碍. 这种天然化合物通过调节巨细胞免疫反应,显示出治疗败血症的前景.

科学领域:

生物化学生物化学
免疫学免疫学免疫学
药理学药理学是指药理学的学科.

背景情况:

败血症涉及到对感染的失调宿主反应,其中巨细胞糖解对免疫恒温至关重要.
向糖溶酶是一种潜在的败血症治疗策略.
东方素 (Ori) 正在研究其在败血症中的抗炎和代谢作用.

研究的目的:

研究Orientin (Ori) 对炎症和新陈代谢在内毒性和败血症的小鼠模型中的影响.
为了探索Ori和糖解酶酸果酸酶肝类型 (PFKL) 之间的相互作用.

主要方法:

在活体研究中,小鼠模型中的内毒性和败血症,以及使用LPS刺激的巨细胞的体外研究.
通过西斑,ELISA和免疫光检测来评估炎症反应.
通过乳酸生产,葡萄糖吸收和ECAR测量来评估糖解活性.
使用CETSA和分子对接,确认Ori-PFKL相互作用.

主要成果:

在败血症小鼠中,Ori的使用改善了生存率,减少了肺损伤,并抑制了细胞因子的释放.
在体外,Ori减弱了LPS诱导的炎症性细胞因子表达,并抑制了糖解.
巨细胞特异性PFKL过度表达取消了Ori的保护作用,证实了PFKL的作用.

结论:

东方药通过直接向PFKL来缓解败血症引起的炎症和代谢功能障碍.
奥里显示出作为治疗败血症治疗的新型治疗剂的潜力.
调节PFKL活动是治疗败血症相关并发症的可行策略.

关键词:

葡萄糖溶解是什么? 葡萄糖溶解巨细胞是一个巨细胞.在这里,ORI是ORI.这就是为什么PFKL.败血症这是一种败血症.

更多相关视频

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Published on: April 3, 2017

Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity

Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity

Published on: June 7, 2017

相关概念视频

A Data-Driven Approach to Quantifying Immune States in Sepsis

A Data-Driven Approach to Quantifying Immune States in Sepsis

This study investigates the immune condition in sepsis by analyzing the quantitative relationships among white blood cells, lymphocytes, and neutrophils in sepsis patients and healthy controls using data visualization analysis and three-dimensional numerical fitting to establish a mathematical model.

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

This protocol allows a researcher to isolate and characterize tissue-resident macrophages in various hallmark inflamed tissues extracted from diet-induced models of metabolic...

Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity

Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity

Macrophages are plastic cells of the hematopoietic system that have a crucial role in protective immunity and homeostasis. In this report, we describe optimized in vitro techniques to phenotypically and functionally characterize graft-infiltrating regulatory macrophages that accumulate in the transplanted organ under tolerogenic...

What is Glycolysis?

What is Glycolysis?

Overview
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...

Outcomes of Glycolysis

Outcomes of Glycolysis

Nearly all the energy used by cells comes from the bonds that make up complex organic compounds. These organic compounds are broken down into simpler molecules, such as glucose. As a result, cells extract energy from glucose over many chemical reactions—a process called cellular respiration.
Cellular respiration can occur aerobically (with oxygen) or anaerobically (without oxygen). In the presence of oxygen, cellular respiration starts with glycolysis and continues with pyruvate...

Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures

Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures

We developed a rat model of severe corneal inflammation through corneal epithelium curettage combined with corneal sutures. The study evaluated corneal inflammation patterns, epithelial proliferation, and changes in limbal stem cells under inflammatory...