相关实验视频

Updated: Jan 20, 2026

Generating Double-Crossover Recombinants of Pseudomonas aeruginosa for Targeted Gene Deletion
02:27

Generating Double-Crossover Recombinants of Pseudomonas aeruginosa for Targeted Gene Deletion

Published on: September 26, 2025

293

在exoU和exoS Pseudomonas aerguinosa中的6型分泌系统效应基因的差异性占有在微生物角质炎中

Tanzina Akter1, Abrar Maswood Haider2, Fiona Stapleton3

  • 1School of Optometry and Vision Science, Faculty of Medicine and Health, University of New South Wales (UNSW), Sydney, NSW-2052, Australia; Microbial Biotechnology Division, National Institute of Biotechnology (NIB), Dhaka-1349, Bangladesh.

Experimental eye research
|January 18, 2026
PubMed
概括

分析了导致角膜感染的Pseudomonas aeruginosa菌株的VI型分泌系统 (T6SS) 基因. 在细胞毒性 exoU 菌株中,pldA 基因明显比侵入性 exoS 菌株更为普遍,这表明它在微生物角质炎的发病过程中发挥了作用.

关键词:
伪omonas空气菌,T3SSSS,这是一个很好的方法.在T6SSSS中使用.在 exoS 上,我们可以使用 exoS.一个 exoUU 的 exoUU.这是pldA.

更多相关视频

Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis08:57

Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis

Published on: March 10, 2014

12.8K
Generation of a Fluorescent Reporter Strain in Pseudomonas aeruginosa03:04

Generation of a Fluorescent Reporter Strain in Pseudomonas aeruginosa

Published on: October 30, 2025

399

相关概念视频

Generating Double-Crossover Recombinants of Pseudomonas aeruginosa for Targeted Gene Deletion02:27

Generating Double-Crossover Recombinants of Pseudomonas aeruginosa for Targeted Gene Deletion

Source: Valentine, M. E., et al. Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection. J. Vis. Exp. (2020).This video demonstrates the generation of double-crossover recombinants in Pseudomonas aeruginosa using a plasmid-based gene deletion strategy. The process involves selecting bacteria that harbor a non-replicative plasmid integrated into the genome via a single crossover and then inducing a second...
293
Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis08:57

Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis

Here we describe a protocol using the mini-himar1 mariner transposon-mediated mutagenesis for generating a high-density insertion mutant library to screen, isolate and identify novel alginate regulators in the prototypic Pseudomonas aeruginosa strain...
12.8K
Generation of a Fluorescent Reporter Strain in Pseudomonas aeruginosa03:04

Generation of a Fluorescent Reporter Strain in Pseudomonas aeruginosa

Source: Rugjee, K. N. et. al., Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa. J. Vis. Exp. (2016)This video demonstrates the generation of a cyclic-di-GMP-responsive reporter strain in Pseudomonas aeruginosa using electroporation. Successful transformation is confirmed through antibiotic selection and fluorescence-based...
399
Biosynthesis of Polyhydroxyalkanoates by Pseudomonas aeruginosa in a Carbon-Rich Medium01:29

Biosynthesis of Polyhydroxyalkanoates by Pseudomonas aeruginosa in a Carbon-Rich Medium

Begin with a strain of Pseudomonas aeruginosa bacteria, isolated from oil-contaminated soil, in a medium containing a nitrogen source and an excess of edible oil as the carbon source.This strain synthesizes polyhydroxyalkanoate (PHA), a long-chain polymer useful in producing bioplastics.Incubate under agitation to facilitate aeration and promote bacterial growth.Initially, the medium supplies both carbon and nitrogen sources to support bacterial proliferation and generate high biomass.After...
124
A Phage Therapy against Pseudomonas aeruginosa Infection in Zebrafish Embryos02:46

A Phage Therapy against Pseudomonas aeruginosa Infection in Zebrafish Embryos

The video demonstrates the phage therapy technique targeting Pseudomonas aeruginosa infection in cystic fibrosis zebrafish embryos. Phage therapy effectively diminishes bacterial load and mitigates proinflammatory immune responses in CF zebrafish embryos infected with bacteria, leading to reduced CF embryo...
502
Swarming Behavior of Pseudomonas aeruginosa Under Antibiotic Stress02:38

Swarming Behavior of Pseudomonas aeruginosa Under Antibiotic Stress

Source: Bru, J., et al., Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response. J. Vis. Exp. (2020)This video demonstrates the procedure for analyzing the swarming behavior of Pseudomonas aeruginosa by observing bacterial growth from a central culture toward satellite spots containing the same culture mixed with an antibiotic, using an agar plate setup and time-lapse...
267