相关实验视频

Updated: Jan 20, 2026

Endoplasmic Reticulum : RER and SER

Endoplasmic Reticulum : RER and SER

在自身免疫性和炎症性疾病中,TNFSF10通过NLRP3炎症体和内质网膜应激驱动过度活跃的免疫反应

Huilin Zhang¹, Yaoyao Chen², Yingmei Li³

¹Translational Medical Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China; Department of Hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

Journal of advanced research

|January 18, 2026

概括

瘤坏死因子连接物超级家族成员10 (TNFSF10) 驱动自身免疫性疾病中的免疫失衡和炎症. 这项研究揭示了TNFSF10的存在.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
病变发生学研究病变发生学研究

背景情况:

自身免疫和炎症性疾病有复杂的原因,需要新的治疗方法.
瘤坏死因子超级家族成员10 (TNFSF10) 在健康和疾病中的确切作用尚未完全理解.

研究的目的:

研究TNFSF10在自身免疫和炎症性疾病中的功能.
阐明TNFSF10影响免疫恒温和细胞死亡的分子机制.

主要方法:

从无形成性贫血 (AA) 患者的免疫细胞的单细胞RNA测序 (scRNA-seq) 确定了TNFSF10作为一个关键的差异表达基因 (DEG).
用TNFSF10缺乏的小鼠来建模AA,以及对内分泌网膜 (ER) 应激,急性移植对宿主疾病 (aGVHD),辐射诱导衰老和炎症性肠病 (IBD) 的模型来探索TNFSF10的生物功能.

主要成果:

发现TNFSF10通过激活NF-κB通路和NLRP3炎症体来破坏免疫平衡,并促进免疫细胞死亡 (ICD).
TNFSF10加剧了ER压力,提高了PPP1R15A的调节,并促进了NLRP3炎症酶激活.
由TNFSF10诱导的ER压力导致表面卡尔雷蒂库林 (CALR) 暴露,信号过度激活的免疫细胞清除造血细胞,和AA特异性的外体可能有助于TNFSF10表达和ER压力.

结论:

TNFSF10在自身免疫和炎症性疾病的发病过程中起着至关重要的作用.
TNFSF10集成NF-κB,NLRP3炎症酶和ER应激通路,加速疾病的进展.

关键词:

无形成性贫血是什么意思自免疫和炎症性疾病.在NLRP3中,NLRP3是NLRP3中的一个.在PPP1R15A中.在TNFSF10中使用.

更多相关视频

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

Published on: April 24, 2021

相关概念视频

Endoplasmic Reticulum

Endoplasmic Reticulum

The Endoplasmic Reticulum (ER) in eukaryotic cells is a substantial network of interconnected membranes with diverse functions, from calcium storage to biomolecule synthesis. A primary component of the endomembrane system, the ER manufactures phospholipids critical for membrane function throughout the cell. Additionally, the two distinct regions of the ER specialize in the manufacture of specific lipids and proteins.

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Dendritic cells (DCs) secrete IL-1β in response to TLR8 recognition of synthetic purine, R848, followed by NLRP3 inflammasome activation with nigericin, therefore, IL-1β can be used to measure NLRP3 inflammasome activity. Intracellular cytokine staining, immunoblotting, and ELISA are used to accurately measure NLRP3 inflammasome priming and activation via IL-1β...

The Endoplasmic Reticulum

The Endoplasmic Reticulum

The endoplasmic reticulum or ER makes up for more than half of the membranes in a cell and accounts for 10% of total cell volume. It is also the primary protein and lipid synthesis factory for most cell organelles, such as the Golgi apparatus, lysosomes, secretory vesicles, and the plasma membrane. Despite being the most extensive and functionally complex subcellular organelle, ER was the last to be discovered. After years of deliberation, Keith Porter and George Palade in the year 1954,...

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

In the present study, the expression is knocked down of two downstream signaling components of the PERK pathway, the cytoprotective calcineurin and the pro-apoptotic CHOP, by using specific shRNAs. In opposite ways, these modulate the susceptibility of primary cortical neurons to neurite atrophy after induction of endoplasmic reticulum stress.

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

We describe the imaging approaches we use to investigate the distribution and mobility of the transfected fluorescent proteins resident in the endoplasmic reticulum (ER) by means of the confocal imaging of living cells. We also ultrastructurally analyze the effect of their expression on the architecture of this subcellular...

Measuring Endoplasmic Reticulum Stress and Unfolded Protein Response in HIV-1 Infected T-Cells and Analyzing its Role in HIV-1 Replication

Measuring Endoplasmic Reticulum Stress and Unfolded Protein Response in HIV-1 Infected T-Cells and Analyzing its Role in HIV-1 Replication

Here, we describe some established methods to determine endoplasmic reticulum (ER) stress and unfolded protein response (UPR) activation, with particular emphasis on HIV-1 infection. This article also describes a set of protocols to investigate the effect of ER stress/UPR on HIV-1 replication and virion...