通过增强的处理和受体亲和力,提高了Vip3Ca蛋白的杀虫活性
Wen Zhang1,2,3, Shen Guo3, Mingyu Xie3
1Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, China.
Pest management science
|January 18, 2026
概括
修改Bacillus thuringiensis Vip3Ca蛋白质改善了其处理和受体结合,显著增强了其杀虫活性,对关键的类虫害如Spodoptera frugiperda.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 昆虫学 昆虫学是一门学科.
背景情况:
- 细菌 thuringiensis Vip3 蛋白质是强大的杀虫剂,可以对抗类虫害.
- 包括Vip3Ca在内的Vip3C亚家族表现出有限的天然杀虫活性.
- 提高Vip3Ca的有效性对于更广泛的害虫控制应用至关重要.
研究的目的:
- 通过增强其处理和受体结合,提高Vip3Ca的杀虫活性.
- 为了设计Vip3Ca突变体,增加对虫虫害的效力.
主要方法:
- 基于Vip3Aa.的构造Vip3Ca蛋白酶分裂部位突变体.
- 引入了特定的残留物替代物 (G302K,N390K,Q468K) 以提高结合亲和力.
- 在S. frugiperda中肠蛋白酶提取物中评估了加工效率.
- 评估了对各种类虫幼虫 (S. frugiperda,H. armigera,S. litura,S. exigua,O. furnacalis) 的杀虫活性.
主要成果:
- 在五种蛋白酶分裂部位突变中,有四种突变表现出对S. frugiperda的加工增加和增强的毒性.
- 选择的突变体显示出对H. armigera,S. litura和S. exigua.的活性有所改善.
- 其余物替代增加了Vip3Ca与S. frugiperda BBMVs的结合亲和力,增加了1.52至1.96倍.
- 组合突变呈现出明显更高的杀虫活性,对O.furnacalis.增加了12.30倍.
结论:
- 优化蛋白质处理和受体结合亲和力有效地增强了Vip3Ca的杀虫活性.
- 改造的Vip3Ca突变体显示显著提高功效,为害虫控制提供了一个有前途的战略.
- 结果为其他Vip3蛋白家族成员的功能优化提供了洞察力.
相关概念视频
06:01Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
7.6K
Here, we present a protocol to screen extracellular protein microarrays for identification of novel receptor-ligand interactions in high throughput. We also describe a method to enhance detection of transient protein-protein interactions by using protein-microbead...
7.6K
11:45Quantifying Agonist Activity at G Protein-coupled Receptors
19.8K
A method for estimating the affinity constant of an agonist for the active state (Kb) of a G protein-coupled receptor is described. The analysis provides absolute or relative measures of Kb depending on whether constitutive receptor activation is measurable. Our method applies to various responses downstream from receptor...
19.8K
08:39Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos
6.1K
A novel technique for the detection of low abundance endogenous receptors present in zebrafish embryos is described. We have named it AFLIP because it consists of affinity labeling of the receptor by its ligand linked to...
6.1K
G-protein Coupled Receptors
131.6K
G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
131.6K
04:43Topical Testing Assay: A Technique to Evaluate the Insecticidal Potential of Dopamine Receptor Antagonists on Adult Female Mosquitoes by Topical Application
659
In this video, we demonstrate the topical testing assay to determine the toxicity of a potential insecticide on adult female mosquitoes. Using a dopamine receptor antagonist that causes lethal inhibition of neurotransmission in mosquitoes, this method describes a way to evaluate the toxic effects of insecticides upon topical...
659
06:30Bioassays for Monitoring Insecticide Resistance
33.9K
This manuscript demonstrates and discusses techniques used to survey pesticide susceptibility and detect resistance to contact and systemic pesticides in arthropod...
33.9K


