相关实验视频

Updated: Jan 20, 2026

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Published on: January 31, 2025

从马体血清和血中隔离细胞外囊泡的比较分析,使用两个隔离方法进行结构和蛋白质学验证

Dominika Milczek-Haduch^1,2, Magdalena Żmigrodzka³, Paula Kiełbik¹

¹Department of Large Animals Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

|January 19, 2026

概括

尺寸排除色谱 (SEC) 提供了一种可复制的方法,用于从马血清中分离细胞外囊泡 (EV),从而产生高质量的生物标志物发现制剂.

科学领域:

兽医医学兽医医学兽医医学
生物化学生物化学
蛋白质组学是指蛋白质组学.

背景情况:

细胞外囊泡 (EVs) 对于细胞间通信至关重要,并具有作为生物标记物的潜力.
从马类生物流体中分离高质量的电动汽车存在重大挑战.
需要标准化的方法来可靠地隔离和分析马的EV.

研究的目的:

为了比较尺寸排除色谱 (SEC) 和微分超离心法,其次是SEC (UC+SEC) 来从马类血清和血中分离EV.
评估通过不同的方法和矩阵获得的电动汽车的效率,可重现性和蛋白质组概况.
确定用于生物标志物发现的马匹EV隔离的最佳工作流程.

主要方法:

马类血清和无血小板血是从六匹健康的马匹中处理的.
使用SEC和UC+SEC工作流程来隔离电动汽车.
描述涉及传输电子显微镜,纳米粒子跟踪分析和基于质谱的蛋白质组学.

主要成果:

与UC+SEC相比,SEC显示出更高的可复制性,蛋白质产量和更简单的工作流.
使用SEC处理的血清产生了与典型的EV标记器最一致的蛋白质组概况.
电动体表现出预期的形态和大小 (100-200纳米);蛋白质含量在血清和血来源之间有所不同.

结论:

选择的隔离方法和生物矩阵显著影响EV产量和蛋白质组合.
对马类血清应用的SEC提供了适合生物标志物发现的可复制,高质量的EV制剂.
这种方法比较突出了影响马匹EV分析的关键因素.

关键词:

外基因组是外基因组的组成部分.细胞外囊泡中的细胞外囊泡.马匹马匹马匹纳米颗粒是一种纳米粒子.血等离子体是什么?血液中的血清是血清.

更多相关视频

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

Published on: October 17, 2022

相关概念视频

Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery

Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery

This article describes a methodology for the isolation, characterization, and quantification of human plasma-derived extracellular vesicles (EV) and presents a workflow for label-free analysis of the EV proteome using liquid chromatography-tandem mass spectrometry...

Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis

Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis

The present protocol provides detailed descriptions for the efficient isolation of urinary extracellular vesicles utilizing functionalized magnetic beads. Moreover, it encompasses subsequent analyses, including western blotting, proteomics, and...

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

Isolation and Analysis of Traceable and Functionalized Extracellular Vesicles from the Plasma and Solid Tissues

The present protocol describes a method to extract extracellular vesicles from the peripheral blood and solid tissues with subsequent profiling of surface antigens and protein...

Polycarbonate Ultracentrifuge Tube Re-Use in Proteomic Analyses of Extracellular Vesicles

Polycarbonate Ultracentrifuge Tube Re-Use in Proteomic Analyses of Extracellular Vesicles

A detailed protocol is provided for cleaning and re-using polycarbonate ultracentrifuge tubes to perform extracellular vesicle isolation suitable for proteomics...

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

This protocol details a method to isolate extracellular vesicles (EVs), small membranous particles released from cells, from as little as 10 μl serum samples. This approach circumvents the need for ultracentrifugation, requires only a few minutes of assay time, and enables the isolation of EVs from samples of limited...

Isolation of Tissue Extracellular Vesicles from the Liver

Isolation of Tissue Extracellular Vesicles from the Liver

This is a protocol to isolate tissue extracellular vesicles (EVs) from the liver. The protocol describes a two-step process involving collagenase perfusion followed by differential ultracentrifugation to isolate liver tissue...