相关实验视频

Updated: Jan 20, 2026

A Broth Microdilution Method for Antibiotic Susceptibility Testing of Gram-Positive Bacteria

A Broth Microdilution Method for Antibiotic Susceptibility Testing of Gram-Positive Bacteria

Published on: August 29, 2025

用中国CAST断点对422个金黄色葡萄球菌分离体进行埃拉瓦环素敏感性测试的磁盘扩散,MIC试卷和微稀释的比较

Xinying Wang^1,2, Xunlu Yin^3,4, Yanjun Liu¹

¹Department of Laboratory Medicine, The Affiliated Taian City Central Hospital of Qingdao University, Taian 271000, China.

The Journal of antimicrobial chemotherapy

|January 19, 2026

概括

磁盘扩散 (DD) 是一种可靠的方法来测试金黄色葡萄球菌对环素的敏感性,与参考微稀释 (BMD) 方法有很好的一致性. 这使得DD成为常规实验室使用的合适替代品.

科学领域:

微生物学微生物学
临床诊断临床诊断临床诊断
抗微生物耐药性抗微生物耐药性

背景情况:

埃拉瓦环素是一种新型抗生素,用于对抗抗性细菌感染.
准确的敏感性测试对于指导有效的环林治疗至关重要.
对临床实验室来说,评估微稀释 (BMD) 的替代方法非常重要.

研究的目的:

评估与 BMD 相比,磁盘扩散 (DD) 和 MIC 试卷 (MTS) 的可靠性,以测试时代环素敏感性.
为了确定DD和MTS的分类协议 (CA),基本协议 (EA) 和错误率.
为了确定测试方法中可能存在的差异,以检测黄金葡萄球菌 (Staphylococcus aureus) 分离物.

主要方法:

测试了422个金黄色葡萄球菌分离物 (256个MSSA,166个MRSA).
汁微稀释 (BMD) 作为参考方法.
作为比较方法,使用了磁盘扩散 (DD) 和MIC试卷 (MTS),并采用了中国CAST标准.

主要成果:

埃拉瓦环素敏感率为93.6% (BMD),96.9% (MTS) 和93.1% (DD).
与BMD相比,磁盘扩散显示出优异的分类一致 (98.1%) 和较低的错误率 (0.7%非常严重的错误).
MIC试验条具有较低的分类一致性 (96.7%) 和较高的非常重大错误 (3.3%),特别是在MRSA分离物中.

结论:

磁盘扩散显示出与BMD在eravacycline敏感性测试中的良好一致性.
DD是一种可靠和合适的替代方法,用于常规实验室检测埃拉瓦环素敏感性.
可能需要对MIC试卷进行进一步评估,特别是对MRSA.

更多相关视频

A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically

A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically

Published on: September 9, 2015

Antibiotic Susceptibility Testing, MIC and Antibiotic Synergy

Antibiotic Susceptibility Testing, MIC and Antibiotic Synergy

Published on: April 30, 2023

相关概念视频

A Broth Microdilution Method for Antibiotic Susceptibility Testing of Gram-Positive Bacteria

A Broth Microdilution Method for Antibiotic Susceptibility Testing of Gram-Positive Bacteria

Source: Koeth, L. M., et. al., A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically. J. Vis. Exp. (2015).This video demonstrates the broth microdilution method for determining the minimum inhibitory concentration (MIC) of a lipoglycopeptide antibiotic against Gram-positive bacteria. It covers bacterial colony isolation, dilution, inoculation of microplate wells with varying antibiotic concentrations, and verification of culture...

A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically

A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically

Here, we present a protocol for determination of dalbavancin susceptibility of clinically relevant Gram-positive bacteria using a broth microdilution reference methodology.

Antibiotic Susceptibility Testing: Epsilometer Tests to Determine MIC Values of Two Antibiotics and Evaluate Antibiotic Synergy

Antibiotic Susceptibility Testing: Epsilometer Tests to Determine MIC Values of Two Antibiotics and Evaluate Antibiotic Synergy

Source: Anna Bläckberg1, Rolf Lood1
1 Department of Clinical Sciences Lund, Division of Infection Medicine, Biomedical Center, Lund University, 221 00 Lund Sweden
Knowledge of the interactions between antibiotics and bacteria is important in understanding how microbes evolve antibiotic resistance. In 1928, Alexander Fleming discovered penicillin, an antibiotic that exerts its antibacterial function by interfering with cell wall regeneration (1). Other antibiotics with diverse mechanisms of...

The Use of Drip Flow and Rotating Disk Reactors for Staphylococcus aureus Biofilm Analysis

The Use of Drip Flow and Rotating Disk Reactors for Staphylococcus aureus Biofilm Analysis

Protocols for utilizing open system flow biofilms with drip flow reactors and rotating disk reactors are presented in...

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

We describe here three different protocols for the in vitro investigation of conjugation, transduction, and natural transformation in Staphylococcus...

Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format

Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format

Antimicrobial susceptibility testing of Mycobacterium tuberculosis is challenging but critical for patient care. This microtiter plate format offers testing of 12 antimycobacterial drugs and provides minimum inhibitory concentration (MIC) values, which will aid in appropriate...