在美洲的Leptospira的宿主驱动的遗传多样性:一个大陆的视角

Alejandro Suárez-Galaz1, Sokani Sánchez-Montes2, Marco Torres-Castro1

  • 1Regional Research Center "Dr. Hideyo Noguchi", Autonomous University of Yucatan, Merida, Mexico, uady.mx.

概括

这项研究揭示了蝙蝠在美洲的遗传多样性更高的Leptospira细菌,而形虫则有助于变种的产生. 主体生态在很大程度上影响了病原体的多样性.

相关概念视频

Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of Leptospira14:49

Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of Leptospira

Here, the application of CRISPR interference (CRISPRi) for specific gene silencing in Leptospira species is described. Leptospira cells are transformed by conjugation with plasmids expressing dCas9 (catalytically "dead" Cas9) and a single-guide RNA (sgRNA), responsible for base pairing to the desired genomic target. Methods to validate gene silencing are presented.
5.7K
Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions05:37

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

Here, we present a protocol to profile the interplay between host and pathogen during infection by mass spectrometry-based proteomics. This protocol uses label-free quantification to measure changes in protein abundance of both host (e.g., macrophages) and pathogen (e.g., Cryptococcus neoformans) in a single...
7.4K
Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples06:05

Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples

In this study, a dot-blot application was designed to detect Leptospira from the three main clades in water samples. This method allows for the identification of minimal DNA quantities specifically targeted by a digoxigenin-labeled probe, easily detected by an anti-digoxigenin antibody. This approach is a valuable and satisfactory tool for screening...
1.8K
Perspectives on Neuroscience26:41

Perspectives on Neuroscience

5.3K
CRISPR Interference-Based Gene Silencing: A Technique for Targeted Repression of Gene Function in Pathogenic Leptospira07:27

CRISPR Interference-Based Gene Silencing: A Technique for Targeted Repression of Gene Function in Pathogenic Leptospira

This video demonstrates a CRISPR interference-based gene silencing in pathogenic Leptospira. Targeted repression of gene expression using a CRISPR/Cas system that blocks mRNA formation helps identify candidate genes for pathological and pharmacological...
3.1K
High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection11:50

High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection

We describe here a technique that combines transposon mutagenesis with high-throughput sequencing to identify and quantify transposon leptospiral mutants in tissues after a challenge of hamsters. This protocol can be used to screen mutants for survival and dissemination in animals and can also be applied to in vitro...
9.3K